| 壳聚糖与牛血清白蛋白作用机制及其复合物乳化性的研究 |
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| 引用本文： | 邱苗,李果,王向阳,黄建颖.壳聚糖与牛血清白蛋白作用机制及其复合物乳化性的研究[J].核农学报,2019,33(5):954-961. |
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| 作者姓名： | 邱苗 李果 王向阳 黄建颖 |
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| 作者单位： | 浙江工商大学食品与生物工程学院/浙江省食品安全重点实验室,浙江杭州 310018;金华职业技术学院制药与材料工程学院,浙江金华321017;浙江工商大学食品与生物工程学院/浙江省食品安全重点实验室,浙江杭州,310018 |
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| 基金项目： | 食品科学与工程浙江省重中之重一级学科(2017SIAR211) |
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| 摘 要： | 为探究蛋白质与多糖之间的相互作用及其复合物的研究乳化性质,本试验以牛血清白蛋白(BSA)-水溶性壳聚糖(WCS)复合体系为研究对象,采用紫外吸收光谱、荧光发射光谱、电位粒径分析、乳液储藏稳定性观察等方法研究在微观环境下蛋白质与多糖的相互作用机理,并进一步探究两者对水包油型乳液乳化性能的影响。结果表明,多糖和蛋白质的相互作用随着pH值和BSA/WCS复合比的变化而不同,混合溶液的紫外吸收峰和荧光发射峰发生偏移,且当复合比小于1∶2时发生荧光淬灭。混合溶液的Zeta电位和平均粒径测定结果表明,BSA与WCS之间主要通过静电相互作用形成复合物,且该复合物的电荷性质、尺寸大小具有pH值和复合比依赖性。研究混合溶液乳化性发现,当pH值为3.0、4.0、复合比为4∶1~1∶1时,形成的乳液液滴粒径较小,性质稳定;当pH 值大于5.0时,乳液液滴粒径大,出现相分离、乳液高度不稳定现象。本研究为设计具有可预测稳定性或刺激响应性的乳液配方及新型功能特性材料提供了理论依据。
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| 关 键 词： | 牛血清白蛋白 壳聚糖 相互作用 乳液 |
| 收稿时间： | 2017-11-27 |
Study of Interaction Mechanism Between Chitosan and Bovine Serum Albumin and Emulsification of Its Complex |
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| QIU Miao,LI Guo,WANG Xiangyang,HUANG Jianying.Study of Interaction Mechanism Between Chitosan and Bovine Serum Albumin and Emulsification of Its Complex[J].Acta Agriculturae Nucleatae Sinica,2019,33(5):954-961. |
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| Authors: | QIU Miao LI Guo WANG Xiangyang HUANG Jianying |
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| Institution: | 1 Food Safety Key Laboratory of Zhejiang Province/School of Food Science and Biotechnology,Zhejiang Gongshang University, Hangzhou, Zhejiang 310018;2 College of Pharmaceutics and Materials Engineering, Jinhua Polytechnic, Jinhua, Zhejiang 321017 |
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| Abstract: | In order to study the interaction between protein and polysaccharide and the emulsification of the complex, the composite system of bovine serum albumin (BSA) and water-soluble chitosan (WCS) was studied in this paper, and the mechanism of protein-polysaccharide interaction in microcosmic environment was investigated by ultraviolet absorption spectrum, fluorescence emission spectrum, potential particle size analysis and emulsion storage stability observation. Furthermore, the effects of BSA and WCS on the emulsification properties of oil-in-water emulsion were studied. The results showed that the interaction between polysaccharide and protein varied with pH value and BSA / WCS ratio. The UV absorption peak and fluorescence emission peak of the mixed solution deviated, and fluorescence quenching occurred when the composite ratio was less than 1:2.The results of Zeta potential and average particle size of the mixed solution showed that the complex was mainly formed by electrostatic interaction between BSA and WCS, and the charge properties and size of the complex were dependent on pH value and composite ratio. The study of emulsification of mixed solution showed that when the pH values were 3.0 and 4.0, and the composite ratios were 4:1-1:1, the droplet size of emulsion droplets was small and the properties were stable. When the pH value was greater than 5.0, the droplet size of the emulsion was large, phase separation occurred, and the emulsion was highly unstable. This study provided a theoretical basis for the design of emulsion formulations with predictable stability or irritability and new functional materials. |
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| Keywords: | bovine serum albumin chitosan interaction emulsion |
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