| 抗除草剂基因基因枪法转化粳稻的研究 |
| |
| 引用本文： | 王景余,金成海,方秀琴,林秀峰,林秀云.抗除草剂基因基因枪法转化粳稻的研究[J].吉林农业科学,2005,30(2):3-6. |
| |
| 作者姓名： | 王景余 金成海 方秀琴 林秀峰 林秀云 |
| |
| 作者单位： | 北京师范大学生命科学学院植物发育生理研究室,北京,100875;吉林省农业科学院水稻研究所,吉林,公主岭,136100;吉林省农业科学院生物技术中心 |
| |
| 摘 要： | 优化了粳稻的组织培养体系;通过Gus基因的瞬间表达研究,明确了以DNA金弹到靶细胞距离为9cm,轰击2次时,PDS1000/He基因枪法转化水稻胚性愈伤组织的效率最高。应用基因枪法将抗除草剂基因(bar)转入吉林省主栽的水稻品种长白8号中,获得了可育的转基因水稻植株。经过Gus基因的组织化学分析,PCR和Southern杂交检测,证明抗除草剂基因已整合到水稻基因组中得以表达,使水稻产生对除草剂的抗性。转基因水稻中发现一例基因沉默现象。
|
| 关 键 词： | 水稻 转基因 抗除草剂 基因枪 |
| 文章编号： | 1003-8701(2005)02-0003-04 |
| 修稿时间： | 2004年11月3日 |
Transformation of Herbicide Resistant Gene to Rice via Biolistic Method |
| |
| Abstract: | A herbicide gene(bar) was delivered into a commercial rice variety "Changbai 8" cultivated in Jilin province via biolistic method. Five fertile transgenic rice plants were obtained. Parameters of Gene Gun(PDS-1000/He) transformation of callus derived from mature embryo of Japonica rice variety were optimized by Gus gene transcient expression. When the distance between DNA-gold pellet and the target cells was 9 cm, bombard for 2 times, the transformation efficiency was the highest. It was demonstrated that bar gene had been inserted into rice genome by PCR and Southern hybridization. Three transgenic rice plants (D0-1, D0-2 and D0-3) showed herbicide resistance. Gene silence was observed in one transgenic rice plant (D0-5). |
| |
| Keywords: | Rice Gene transformation Herbicide resistant Biolistic |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
