Differences in immune responses of pigs vaccinated with Salmonella Typhimurium and S. Choleraesuis strains and challenged with S. Choleraesuis |
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| Institution: | 1. Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, 602 Tower Rd., Ithaca, NY, 14853, United States;2. Department of Microbiology, Immunology, and Biopharmaceuticals, College of Life Sciences, National Chiayi University, No. 300, University Rd., Chiayi, 60004, Taiwan, ROC;3. Department of Animal Sciences, College of Agriculture, National Chiayi University, No. 300, University Rd., Chiayi, 60004, Taiwan, ROC;1. Molecular Medicine Department, Medical Institute, National Institute of Genetics and Biotechnology, Tehran, Iran;2. Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran;3. Pertussis Reference Laboratory? Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran;4. Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran;5. Research Center of Pediatrics Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran;1. Department of Veterinary Parasitology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences university, Ludhiana, 141004, Punjab, India;2. Punjab Remote Sensing Centre, Ludhiana, Punjab, India;1. Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil;2. Laboratório de Bacteriologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil |
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| Abstract: | S. Choleraesuis (Choleraesuis) and S. Typhimurium (Typhimurium) cause salmonellosis in pigs and humans. The effects of vaccine strains pSV-less Typhimurium OU5048 and Choleraesuis OU7266 and SPI-2-mutant Choleraesuis SC2284 on the immune responses of pigs against Typhimurium, Choleraesuis, and S. Enteritidis (Enteritidis) with or without the virulence plasmid (pSV) were determined. After oral vaccination of three vaccine groups and challenge with Choleraesuis CN36, the level of Salmonella-specific IgG in sera and the bactericidal effects and superoxide generation of peripheral blood mononuclear cells (PBMCs) and polymorphonuclear leukocytes (PMNs) against the above strains were determined using ELISA and NBT assay, respectively. Among three vaccine strains tested, OU7266 stimulated the highest Salmonella-specific IgG levels. Complement inactivation increased IgG concentration, while E. coli absorption reduced IgG levels. The pSV-containing strains were less resistant to serum killing than the pSV-less strains, and Enteritidis exhibited the lowest resistance to serum killing. Serovars tested, vaccine strains, and timeline periods postvaccination and challenge were important factors affecting superoxide production. The two Choleraesuis vaccine strains stimulated greater levels of superoxide from PMNs and PBMCs than the Typhimurium strains. The PMNs and PBMCs in challenged and vaccinated pigs reduced more superoxide than those in challenged hosts. In vaccinated hosts, pSV-less Salmonella strains triggered lower levels of PMN/PBMC-generated superoxide upon challenge than strains with pSV against Enteritidis and Choleraesuis. Overall, Choleraesuis OU7266 may be better than the other vaccine strains in generating the greatest IgG levels, serum bactericidal activity and superoxide levels. The pSV likely influences the immune responses. |
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| Keywords: | Antibody Pig Serum resistance Superoxide Vaccine Virulence plasmid |
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