Prevalence of Coxiella burnetii in milk collected from buffalo (water buffalo) and cattle dairy farms in Northwest of Iran |
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| Institution: | 1. Programa de Investigación en Medicina Poblacional, Escuela de Medicina Veterinaria, Universidad Nacional (UNA), P.O. Box 86-3000, Heredia, Costa Rica;2. Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals with Veterinary Ambulance, Faculty of Veterinary Medicine, Justus Liebig University Giessen, 35392 Giessen, Germany;3. Institute of Parasitology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, 35392 Giessen, Germany |
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| Abstract: | The present study was conducted to determine the prevalence of C. burnetii in raw milk samples collected from water buffalos and cattle in Northwest of Iran (West Azerbaijan Province). A total number of 840 milk samples were randomly collected from buffalos and cattle belonged to three different geographical regions in west Azerbaijan (the map is necessary). The milk samples were collected seasonally during 2018 and the age of animals were recorded. All the milk samples were subjected to DNA extraction. Nested-PCR was used to detect C. burnetii based on the transposable gene IS1111. The results showed that 16.9% (95% CI: 14.5%–19.6%) of the examined milk samples (19.3% buffalo and 14.6% cattle samples) were positive for C. burnetii. There was a significant difference in C. burnetii shedding in milk between different age groups in cattle but not in buffalos (p value <0.05). The shedding of C. burnetii in milk was highly prevalent in summer (31.1%) (p < 0.05, 95% CI: 26.1%–38.4%). There were significant regional and seasonal variations in the prevalence of C. burnetii in the examined milk samples. It was concluded that buffalo population in west Azerbaijan should be considered as an important factor in the epidemiology of Q fever and consequently in public health. |
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| Keywords: | Nested-PCR Cattle milk Buffalo milk West Azerbaijan |
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