High-resolution melt curve analysis: A real-time based multipurpose approach for diagnosis and epidemiological investigations of parasitic infections |
| |
| Institution: | 1. Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran;3. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran;1. Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Department of Biostatistics, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran;3. Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran;4. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran;5. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran;1. School of Animal and Veterinary Sciences, Faculty of Science, Charles Sturt University, NSW 2678, Australia;2. School of Biomedical Sciences, Faculty of Science, Charles Sturt University, Wagga Wagga, NSW 2650, Australia;1. Molecular Virology Unit, Virology Division, Kimron Veterinary Institute, Bet-Dagan, 50250, Israel;2. German Research Center for Artificial Intelligence, Knowledge Management Group, Berlin, Germany;3. HaChaklait, P.O. Box 3039, Caesarea, Israel |
| |
| Abstract: | BackgroundReal-time PCR coupled with high resolution melting curve analysis is a practical technique that could be employed in multipurpose studies. During the recent decade, this technique has been practiced for different targets, worldwide.MethodsIn the current study three major database centers consisted of PubMed, Scopus and Web of Science were searched until Aug 2019 for applications of HRM real-time PCR in parasitology studies using terms: “Parasite” AND “HRM real-time PCR” OR “High Resolution Melting curve analysis” OR “Real-time PCR”, “Protozoan parasites” AND “HRM real-time PCR” OR “High Resolution Melting curve analysis” OR “Real-time PCR”, “Helminth” AND “HRM real-time PCR” OR “High Resolution Melting curve analysis” OR “Real-time PCR”.ResultsTotally, 83 papers met our criteria and were included in our study. This method was more frequently used for protozoan parasites (52/83; 62.65%), while lower (31/83; 37.35%) studies were incorporated on helminths parasites. Furthermore, Plasmodium spp., and Leishmania spp., were the most prevalent protozoan parasites, and Taenia spp., and filers were the most frequent helminths that were studied by HRM real-time PCR.ConclusionHRM real-time PCR is a sensitive, flexible and cost-effective method that could be used for multipurpose studies. |
| |
| Keywords: | Molecular diagnosis HRM real-time PCR Parasitic infection Multipurpose method Systematic review |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
