Brucella canis induces canine CD4+ T cells multi-cytokine Th1/Th17 production via dendritic cell activation |
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| Institution: | 1. Program of Immunology, Institute of Biomedical Sciences ICBM, Faculty of Medicine, Universidad de Chile, Santiago, Chile;2. Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, Santiago, Chile;3. Laboratory of Veterinary Bacteriology, Department of Animal Preventive Medicine, Faculty of Veterinary Sciences, Universidad de Chile, Santiago, Chile;4. Centro de Investigaciones Biológicas (CIB), Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain;5. Dentistry Unit, Faculty of Health Sciences, Universidad Autónoma de Chile, Santiago, Chile;1. UNL, Biotechnological Development Laboratory, FBCB, Edificio FBCB-Ciudad Universitaria UNL, C.C. 242. (S3000ZAA), Santa Fe, Argentina;2. UNL, CONICET, Cell Culture Laboratory, FBCB, Edificio FBCB-Ciudad Universitaria UNL, C.C. 242. (S3000ZAA), Santa Fe, Argentina;3. Cellargen Biotech S.R.L. Antonia Godoy 6369, C.C. 242. (S3000ZAA), Santa Fe, Argentina;1. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China;1. Laboratorio de Inmunología, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), Centro de Investigación Veterinaria Tandil (CIVETAN-CONICET-CICPBA), Facultad de Ciencias Veterinarias (FCV), Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Tandil, 7000, Buenos Aires, Argentina;2. Departamento de Farmacia. Facultad Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA-CONICET), Universidad Nacional de Córdoba, Argentina;3. Laboratorio de Bacteriología, Departamento de Producción Animal, Instituto Nacional de Tecnología Agropecuaria, Balcarce, 7620, Argentina;4. Área de Epidemiología. SAMP. CIVETAN-CONICET-CICPBA, FCV, UNCPBA, Tandil, Buenos Aires, Argentina;5. Área de Cirugía. Depto. Clínica. CIVETAN-CONICET-CICPBA, FCV, UNCPBA, Tandil, Buenos Aires, Argentina;6. Inmunova S.A., Buenos Aires, Argentina;7. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina;1. Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO, 65211, United States;2. Laboratory for Infectious Disease Research, University of Missouri, Columbia, MO, 65211, United States;1. Polytechnic Institute of Castelo Branco, School of Agriculture, Castelo Branco, Portugal;2. QRural – Quality of Life in the Rural World – Research Unit, Polytechnic Institute of Castelo Branco, Castelo Branco, Portugal;3. CERNAS – Research Center for Natural Resources, Environment and Society, Polytechnic Institute of Castelo Branco, Castelo Branco, Portugal;4. CECAV – Animal and Veterinary Research Centre, Department of Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal;5. Municipality of Mação, Mação, Portugal;6. CIISA – Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisboa, Portugal;7. Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, Institute of Parasitology, University of Bern, Bern, Switzerland;8. Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM). University of Évora, Núcleo da Mitra, Évora, Portugal;1. Department of Biostatistics, Epidemiology, and Informatics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States;2. Zoonotic Disease Research Lab, One Health Unit, School of Public Health and Administration, Universidad Peruana Cayetano Heredia, Lima, Lima Province, Peru;3. Department of Biology, University of Pennsylvania, Philadelphia, PA, United States;4. Department of Preventive Medicine and Public Health, Facultad de Medicina San Fernando, Universidad Nacional Mayor de San Marcos, Lima, Peru |
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| Abstract: | Brucella canis is a small intracellular Gram-negative bacterium that frequently leads to chronic infections highly resistant to antibiotic therapy in dogs. Also, it causes mild human brucellosis compared to other zoonotic Brucella spp. Herein we characterize the cellular immune response elicited by B. canis by analysing human and canine CD4+ T cells after stimulation with autologous monocyte-derived dendritic cells (MoDCs). Human and canine B. canis-primed MoDCs stimulated autologous CD4+ T cells; however, a Th1 response was triggered by human MoDCs, whereas canine MoDCs induced Th1/Th17 responses, with increased CD4+ T cells producing IFN-γ and IL-17A simultaneously. Each pattern of cellular response may contribute to host susceptibility, helping to understand the differences in B. canis virulence between these two hosts. In addition, other aspects of canine immunology are unveiled by highlighting the participation of IL-17A-producing canine MoDCs and CD4+ T cells producing IFN-γ and IL-17A. |
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| Keywords: | Dendritic cells Host susceptibility Cytokines |
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