Genetic diversity of Streptococcus equi subsp. zooepidemicus isolated from horses |
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| Institution: | 1. Aix-Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France;2. Department of Epidemiology and Health Economics, Aix-Marseille Univ, Marseille, France;3. APHM, IHU Méditerranée Infection, UF Immunologie, Marseille, France;1. Department of Animal Management, College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;2. Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Muñoz 3120, Nueva Ecija, Philippines;3. Department of Biological Sciences, College of Arts and Sciences, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;4. Department of Animal Science, College of Agriculture, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;1. Universidade Federal Fluminense, Departamento de Microbiologia e Parasitologia, Laboratório de Bacteriologia Veterinária, Rua Professor Hernani Melo 101, Niterói, 24210-130, Brazil;2. Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Laboratório de Epidemiologia Molecular e Resistência a Antimicrobianos, Av. Prof. Orlando Marques de Paiva 87, São Paulo, 05508-010, Brazil;3. Universidade Federal de Pelotas, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Rua Gomes Carneiro 1, Pelotas, 96010-610, Brazil;1. INMIVET, Department of Animal Health, Faculty of Veterinary Science, Complutense University of Madrid, 28040 Madrid, Spain;2. LeishmanCeres Laboratory (GLP Compliance Certificated), Parasitology Unit, Veterinary Faculty, University of Extremadura, Avenida de la Universidad s/n, 10003, Cáceres, Spain;3. Hospital Central de la Defensa Gómez Ulla, 28047 Madrid, Spain;1. Departamento de Sanidad Animal, Facultad de Veterinaria, UCO, Campus Universitario de Rabanales, 14071 Córdoba, Spain;2. Grupo de Sanidad y Biotecnología, Instituto de Investigación en Recursos Cinegéticos, Universidad de Castilla la Mancha, (SaBio-IREC, UCLM-CSIC-JCCM), 13005, Ciudad Real, Spain;3. Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, UCO, Campus Universitario de Rabanales, 14071 Córdoba, Spain;4. Unidad de Enfermedades Infecciosas, Grupo de Virología Clínica y Zoonosis, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Hospital Universitario de Córdoba, 14004 Córdoba, Spain;5. IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain |
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| Abstract: | Streptococcus equi subsp. zooepidemicus (SEZ) is an opportunistic and zoonotic pathogen of horses. In this study, genetic intraspecies variability of SEZ obtained mainly from respiratory and genital samples of horses was investigated by analysis of the 16S–23S rRNA intergenic spacer region (ISR) and of the 16S rRNA gene. 16S–23S ISR rRNA type A1 was predominant, although a high rate of multiple products (30.5%) was obtained. Phylogenetic analysis of the 16S rRNA gene detected three genogroups (I, II and III). 16S rRNA variable regions V1 and V2 are the most important regions for evaluating SEZ intraspecies variability, but at least V1-V5 regions should be considered to avoid mistakes. Analysis of all 16S rRNA sequences available in databases assigned human SEZ to groups I and III but not to group II. These results show a high genetic variability in SEZ collected from different specimens of horses from various regions of Italy. |
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| Keywords: | Horses Genotyping 16S-23S rRNA intergenic spacer region 16S rRNA |
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