| 抑制消减杂交联合基因芯片技术及其在动物基因差异表达研究中的应用 |
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| 引用本文： | 姜俊芳,宋雪梅,蒋永清.抑制消减杂交联合基因芯片技术及其在动物基因差异表达研究中的应用[J].畜牧与饲料科学,2011(8):57-59. |
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| 作者姓名： | 姜俊芳 宋雪梅 蒋永清 |
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| 作者单位： | 浙江省农业科学院畜牧兽医研究所,浙江杭州,310021 |
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| 基金项目： | 浙江省自然科学基金项目(Y307045); 浙江省农业科学院科研类专项经费资助项目(2010R22Y01D02) |
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| 摘 要： | 抑制消减杂交技术(SSH)与基因芯片技术是研究差异表达基因的两种不同的方法,这两种技术如果单独使用都会存在不同程度的不足。近年来,研究人员发现,将SSH和基因芯片技术结合使用,可充分发挥各自的优势,弥补各自的不足,实现差异表达基因的大规模高效筛选,因此,抑制消减杂交联合基因芯片技术成为目前分析差异表达基因新的有效手段。介绍了SSH技术、基因芯片技术以及两者结合的原理与优缺点,同时概述了抑制消减杂交联合基因芯片技术在动物差异表达基因研究中的应用。
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| 关 键 词： | 抑制消减杂交技术 基因芯片技术 差异表达基因 |
Application of Suppression Subtractive Hybridization Combining with Gene Chip Technology in Study on Animal Differentially Expressed Genes |
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| JIANG Jun-fang,SONG Xue-mei,JIANG Yong-qing.Application of Suppression Subtractive Hybridization Combining with Gene Chip Technology in Study on Animal Differentially Expressed Genes[J].Animal Husbandry and Feed Science,2011(8):57-59. |
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| Authors: | JIANG Jun-fang SONG Xue-mei JIANG Yong-qing |
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| Institution: | JIANG Jun-fang,SONG Xue-mei,JIANG Yong-qing(Research Institute of Animal Husbandry and Veterinary,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China) |
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| Abstract: | Suppression subtractive hybridization(SSH) and gene chip technology are two different methods to study differentially expressed genes,but if the two technologies are used individually,they will have their own shortages at different degree.Recently,scholars have found that if SSH is combined with gene chip technology,the two technologies could play their own advantages and make up their own disadvantages so as to realize the large-scale efficient screening of differentially expressed genes,so SSH combining w... |
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| Keywords: | suppression subtractive hybridization(SSH) gene chip technology differentially expressed genes |
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