| 芥蓝薄细胞层培养中愈伤组织和器官形成的细胞组织学观察 |
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| 引用本文： | 郑玉梅,李育.芥蓝薄细胞层培养中愈伤组织和器官形成的细胞组织学观察[J].华南农业大学学报,1994,15(4):18-22. |
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| 作者姓名： | 郑玉梅 李育 |
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| 作者单位： | 广州华南农业大学农业生物系，江西省供销社工业贸易公司工作 |
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| 摘 要： | 芥蓝花序轴的薄细胞层，培养在附加０．５ｍｇ／ＬＮＡＡ，５ｍｇ／ＬＢＡ以及０．２５ｇ／Ｌ水解乳蛋白的ＭＳ培养基础上，诱导出愈伤组织并进一步形成芽和根，显微镜检表明，表皮和亚表皮都可进行细胞分裂，分生细胞团起源于亚表皮，器官发生有直接发生和间接发生类，外植株细胞未脱分化时，无淀粉累积，一旦分化启动，便累积淀粉，随细胞增殖及芽的分化，细胞中累积的淀粉被利用而消失，当芽原基形成之后，又积累淀粉。
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| 关 键 词： | 芥蓝 薄细胞层 愈伤组织 器官形成 |
CYTOHISTOLOGICAL OBSRVATIONS ON THE CALLUSFORMATION AND ORGANOGENESIS IN THIN CELLLAYER CULTURES OF RACHES OF Brassica alboglabra BAILEY |
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| Zheng Yumei,Li Yu,Wang Shimao.CYTOHISTOLOGICAL OBSRVATIONS ON THE CALLUSFORMATION AND ORGANOGENESIS IN THIN CELLLAYER CULTURES OF RACHES OF Brassica alboglabra BAILEY[J].Journal of South China Agricultural University,1994,15(4):18-22. |
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| Authors: | Zheng Yumei Li Yu Wang Shimao |
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| Abstract: | The thin cell layers from the raches of B. alboglabra Bailey were cultured onMS medium(MS +5 mg/L NAA + 5 mg/L BA + 0.25 g/L LH). After 6 days of cuIture,calli grew formthe explants, and then regenerated shoots and roots were formed fromthese calli. Both epidermal and subepidermal cells may be activated to divide. Themeristematic eellular masses were derived from the subepidermal cells.Two types oforganogenesis occured:direct and indirect organogenesis. Cytochemical observationsshowed that there was no accumulation of starch in the cells of explants before celldedifferentiation. The starch accumulated after differentiation was initiated. With themultiplication of cells and bud regeneration, the starch in the cells was consumed anddisappeared. But the starch accumulated again,when the bud primordia were formed. |
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| Keywords: | thin cell layers Brassica alboglabra callus organogenesis |
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