| 高效液相色谱法测定蜂蜜中β-呋喃果糖苷酶酶活 |
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| 引用本文： | 王介平,郭军,陈杨,王海燕,李睿,王永亮.高效液相色谱法测定蜂蜜中β-呋喃果糖苷酶酶活[J].蜜蜂杂志,2011,31(12):21-23. |
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| 作者姓名： | 王介平 郭军 陈杨 王海燕 李睿 王永亮 |
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| 作者单位： | 重庆市畜牧科学院,重庆荣昌,402460 |
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| 摘 要： | 建立了一种检测蜂蜜中β-呋喃果糖苷酶酶活的方法，即样液中加棉籽糖作为反应底物，在pH为5．4的缓冲液中，45aC摇床200r／min条件下反应2h，测定样液中的密二糖含量。色谱条件为：Phenomenex NH2 Luan 5U 250×4．60mm；流动相：乙腈：水=80：20；进样量：10μL；流速：1．2mL／min；柱温：40℃；示差折光检测器，温度40cc。实验结果表明：β-呋喃果糖苷酶酶活在0．3—1．7U／mL范围内线性良好，相关系数R为0．998 10。相对标准偏差为1．72％（n=5），平均回收率为94．5％。该分析方法简便、准确、灵敏，重现性好，适用于蜂蜜中β-呋喃果糖苷酶酶活的检测。
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| 关 键 词： | 掺假蜂蜜 液相色谱法（HPLC） β-呋喃果糖苷酶 棉籽糖 |
Assay of Beta-fructofuranosidase Activity in Honey by High Cerformance Liquid Chromatography |
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| WANG Jie-ping,GUO Jun,CHEN Yang,WANG Hai-yang,LI Rui,WANG Yong-liang.Assay of Beta-fructofuranosidase Activity in Honey by High Cerformance Liquid Chromatography[J].Journal of Bee,2011,31(12):21-23. |
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| Authors: | WANG Jie-ping GUO Jun CHEN Yang WANG Hai-yang LI Rui WANG Yong-liang |
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| Institution: | WANG Jie-ping,GUO Jun,CHEN Yang,WANG Hai-yang,LI Rui,WANG Yong-liang(ChongQing Academy of Animal Sciences,Rongchang,Chongqing 402460,China) |
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| Abstract: | We analyzed the concentration of melibiose in the sample solution,which reacted 2h after add raffinose as reaction substrate （reaction conditions： pH 5.4 buffer solution,45℃ shaking table 200r/min）.At last,A method was developed for the determination of beta-fructofuranosidase activity in honey. The sample was separated with NH2 column （250 ×4.60mm, 5u） at 40℃, which was then eluted with 80% acetonitrile aqueous solution at a rate of 1.2mL/min,and the signal was detected by RID.The results showed that the linear range for beta-fructofuranosidase activity were in the range of 0.3-1.7 u/mL with a correlation coeficient of 0.998 10. The relative standrd deviations for beta-fructo- furanosidase activity were 1.72% （n=5） and the average recoveries were 94.5%.This method is simple, accurate, sensi- tive and better reproducibility. |
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| Keywords: | honey adulterated high performance liquid chromatography beta-fructofuranosidase raffinose |
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