| 海涂互花米草与水稻远缘杂交种质资源发掘与创新(Ⅳ)(英文) |
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| 引用本文： | 陈启康,田曾元,沙文锋,顾拥建,戴晖,朱娟.海涂互花米草与水稻远缘杂交种质资源发掘与创新(Ⅳ)(英文)[J].农业科学与技术,2012(1):131-133,172. |
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| 作者姓名： | 陈启康 田曾元 沙文锋 顾拥建 戴晖 朱娟 |
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| 作者单位： | 江苏沿江地区农业科学研究所;郑州大学生物工程系 |
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| 基金项目： | Supported by National Natural Science Foundation of China(31072064);Foundation Project of Self-reliant Innovation for Agricultural Science and Technology,Supporting Project for Exploration and Research of Jiangsu Province in2011(TCX(11)4133,CX(11)4060)~~ |
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| 摘 要： | 目的]发掘互花米草耐盐、水稻高产种质资源,创制海涂粮饲兼用耐盐水稻新种质。方法]于2009-2011年开展海涂互花米草与水稻远缘杂交研究,采用海涂互花米草与水稻属间远缘杂交育种方法,同时采用四选一突破结合技术--海涂种植筛选、细胞学检测筛选、回交表型筛选、分子标记辅助筛选结合技术,寻找海涂粮饲兼用耐盐水稻新种质。结果]2009~2010年实际远缘杂交成功率为1.39%。7C14、中香1号2种水稻母本与互花米草(H)父本远缘杂交材料进行RAPD分子鉴定,发现RH-1-10K205-7C14×H、RH-2-8K157-7C14×H、RH-13-9H5-中香1号×H具有与互花米草亲本相同的条带,而同时在水稻亲本中缺失的情形,表明以上远缘杂交种具有互花米草亲本遗传成分;水稻7K339母本与互花米草父本远缘杂交材料经RAPD分子鉴定,杂交种RH-5-10K215、RH-6-8K48、RH-12-9H9、RH-14-9H8、RH-16-9H28具有与互花米草亲本相同的条带,而同时在水稻亲本中缺失的情形,表明以上远缘杂交种具有互花米草亲本遗传成分。其余杂交种与互花米草亲本和水稻亲本也存在不同程度的变异。结论]育出耐盐优质饲料稻新品种,不但解决了沿海开发、盐碱地治理中急需解决的耐盐作物品种问题,而且解决了草食动物精粗饲料问题,同时对于资源利用、农业增效、粮食安全、耕地战略等方面有着重要的科学意义,具有广阔的应用前景。
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| 关 键 词： | 互花米草/水稻 远缘杂交 新种质创制 |
Exploration and Innovation of Distant Hybridization Germplasm of Oryza sativa and Spartina alterniflora |
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| Qikang CHEN,Zengyuan TIAN,Wenfeng SHA,Yongjian GU,Hui DAI,Juan ZHU.Exploration and Innovation of Distant Hybridization Germplasm of Oryza sativa and Spartina alterniflora[J].Agricultural Science ＆ Technology,2012(1):131-133,172. |
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| Authors: | Qikang CHEN Zengyuan TIAN Wenfeng SHA Yongjian GU Hui DAI Juan ZHU |
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| Institution: | 1. Institute of Agricultural Sciences in coastal areas of Jiangsu, Nantong Association for industrialization of Sheep industry, Rugao 226541, China; 2. Bio-engineering Department of Zhengzhou University, Zhengzhou 450052, China |
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| Abstract: | Objective] The aim was to explore salt-resistant Spartina alterniflora and high-yield rice with germplasm resource in order to create new salt-resistant rice species with dual-purpose of food and forage. Method] From 2009 to 2011, researches on distant hybridization of Spartina alterniflora and Oryza Sativa have been conducted with method of distant hybridization breeding. On the other hand, breakthrough and combination techniques (four selected one) were adopted to find the new rice species, including techniques of planting and selection in seashore, cytological detection and selection, phenotype selection of backcross, and molecular marker assisted selection. Result] Success rate of distant hybridization from 2009 to 2010 was 1.39%. RAPD molecular identification of 7C14 and rice female parent of Zhongxiang No.1, seed 2 was carried on with distant hybridization of male parent of Spartina alterniflora (H). The results showed that bands same with Spartina alterniflora parents were found in RH-1-10K205-7C14×H, RH-2-8K157-7C14×H, and RH-13-9H5-Zhongxaing No.1×H. Considering absent situation in rice parents, distant hybridization strains above were found with same parental genetic element as Spartina alterniflora. Female parent of rice 7K339, however, was under RAPD molecular identification with male parent of Spartina alterniflora. The result showed that bands same with Spartina alterniflora parents were found in RH-5-10K215, RH-6-8K48, RH-12-9H9, RH-14-9H8 and RH-16-9H28. Considering absent situation in rice parents, distant hybridization strains above were found with same parental genetic element as Spartina alterniflora. Rest hybrid strains were found variance with Spartina alterniflora parents and rice parents in varying degrees. Conclusion] New and excellent rice species of dual-purpose solves problem not only about salt-resistant species badly needed in coastal development and treatment of saline and alkaline land, but about fine fodder and roughage for herbivore, as well. What’s more, this is of scientific significance in recourse utilization, efficiency improvement in agriculture, food security, and cultivation strategy. |
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| Keywords: | Spartina alterniflora/Oryza sativa Distant hybridization Innovation of new species |
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