| High Level Expression of Glucose-6-phosphate Dehydrogenase Gene PsG6PDH from Populus suaveolens in E. coli |
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| 作者单位： | Lin Yuan-zhen Zhang Zhi-yi* Lin Shan-zhi Zhang Qian Wang Xin Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants，Ministry of Education，Beijing Forestry University，Beijing 100083，P. R. China |
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| 摘 要： | In order to investigate the functions of the gene PsG6PDH and the mechanisms underlying freezing tolerance of Populus suaveolens, the recombinant expression vector pET-G (pET30a-G6PDH), which contained full encoding region of PsG6PDH gene, was established. The recombinant was identified by lawn-PCR and double enzyme digestion and then transformed into expression host XA90 and induced by isopropyl-a-D-thiogalactoside (IPTG) to express 100 kD polypeptide of G6PDH fusion protein. The results showed that the expressed amount of the fusion protein culminated after 1 mmol·L–1 IPTG treatment for 4 h and that pET-G product was predominately soluble and not extra-cellular secreting.
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High Level Expression of Glucose-6-phosphate Dehydrogenase Gene PsG6PDH from Populus suaveolens in E. coli |
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| Authors: | Lin Yuan-zhen Zhang Zhi-yi Lin Shan-zhi Zhang Qian Wang Xin Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants Ministry of Education Beijing Forestry University Beijing P R China |
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| Institution: | Lin Yuan-zhen Zhang Zhi-yi* Lin Shan-zhi Zhang Qian Wang Xin Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,Beijing 100083,P. R. China |
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| Abstract: | |
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| Keywords: | Populus suaveolens glucose 6-phosphate dehydrogenase PsG6PDH prokaryotic expression |
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