| 大田条件下三七光合色素、酚类和总皂苷含量对增补UV-B的响应 |
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| 引用本文： | 赵昶灵,;陈中坚,;魏富刚,;李孙文,;杨莉,;李俊,;王崇德.大田条件下三七光合色素、酚类和总皂苷含量对增补UV-B的响应[J].农业科学与技术,2014(7):1120-1124. |
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| 作者姓名： | 赵昶灵 ;陈中坚 ;魏富刚 ;李孙文 ;杨莉 ;李俊 ;王崇德 |
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| 作者单位： | [1]云南农业大学农学与生物技术学院,云南昆明650201; [2]文山市苗乡三七实业有限公司,云南文山663000; [3]文山学院三七研究所,云南文山663000; [4]云南农业大学农科专业基础实验教学中心,云南昆明650201; [5]云南农业大学植物保护学院,云南昆明650201 |
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| 基金项目： | 国家自然科学基金（31060045,31260091）. |
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| 摘 要： | 目的]探究大田中增补UV-B胁迫条件下1年生三七植株光合色素、酚类（包括总酚、总黄酮和花色苷）和总皂苷的含量变化及其相关性.方法] 1年生三七植株在大田中每天被UV-B辐射1 min,在UV-B灯下的植株被作为圆心,实现叶片的环状取样;叶片光合色素、酚类和总皂苷含量用分光光度法检测.结果]随着取样半径的增加,增补UV-B的强度显著下降,叶片叶绿素（Chl）a、b、Chl （a＋b）、类胡萝卜素（Car）和总光合色素（Chl＋Car）极显著增加,Chl a/b总酚含量（TPC）极显著下降,但Chl （a＋b）/Car变化不显著.总黄酮、总花色苷和总皂苷含量均因UV-B强度的增加而增加.UV-B强度与Chl a/b呈正相关,与Chl a、Chl b、Chl（a＋b）、Car和（Chl＋Car）含量呈负相关;TPC、总黄酮含量（TFC）、总花色苷含量（TAC）和总皂苷含量（TSC）两两间呈正相关,且均达到极显著水平;UV-B强度与TFC呈显著正相关、与Chl （a＋b）/Car呈显著负相关、与TPC、TAC和TSC间呈不显著正相关.结论]合适剂量的增补UV-B可能是提高三七TSC的有效措施之一.
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| 关 键 词： | 1年生三七植株 光合色素 酚类和总皂苷含量 增补UV-B 响应 |
Responses of Photosynthetic Pigment,Phenol and Total Saponin Contents of Panax notoginseng to Supplemental UV-B under Field Conditions |
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| Institution: | Changling ZHAO, Zhongjian CHEN, Fugang WEI, Sunwen LI, Li YANG, Jun LI, Chongde WANG(1.College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China; 2.Miaoxiang Sanqi Industrial Corporation Ltd. of Wenshan City, Wenshan 663000, China;3. Sanqi Research Institute, Wenshan University, Wenshan 663000, China; 4.Teaching Center of the Basic Experiments of Agricultural Majors, Yunnan Agricultural University, Kunming 650201, China;5. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China) |
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| Abstract: | Objective] The aim of this study was to investigate the content changes and their correlations of the photosynthetic pigment,phenols,including total phenols,total flavonoids and anthocyanins,and total saponins of the one-year-old P.notoginseng plants under supplemental UV-B stress in fields.Method] The one-year-old plants were irradiated by UV-B in field for 1 min per day,and the plants under the UV-B lamp were regarded as a circle center,achieving an annular leaf-sampling.The photosynthetic pigment,phenols and total saponins of the leaves were determined spectrophotometrically.Result] With the increase of sampling radius,the supplemental UV-B intensity decreased significantly,the contents of chlorophyll （Chl） a,Chl b,Chl （a＋b）,carotenoid （Car） and total photosynthetic pigment （Chl＋Car） of the leaves increased extremely significantly,the Chl a/b and total phenol content （TPC） decreased extremely significantly,but the Chl （a＋b）/Car changes were not significant.The contents of total flavonoids,anthocyanins and saponins all increased due to the increasing of UV-B,displaying dose effects.The UV-B intensity was positively correlated with the Chl a/b,and negatively with the Chl a,Chl b,Chl （a＋ b）,Car and （Chl＋Car） contents; and the two of TPC,total flavonoid content （TFC）,total anthocyanin content （TAC） and total saponin content （TSC） were positively correlated,all reaching extremely significant level.The UV-B intensity was positively and significantly correlated with the total flavonoid content （TFC）,negatively and significantly with the Chl （a＋b）/Car,and positively and insignificantly with the TPC,TAC and TSC.Conclusion] For one-year-old plants of P.notoginseng,UV-B can decrease the contents of the Chl a,Chl b,Chl （a＋b）,Car and （Chl＋Car） and increase the Chl a/b and TPC,and,furthermore,induce the increases of the TFC,TAC and TSC in a dose-dependent manner.However,UV-B can hardly change the Chl （a＋b）/Car.The supplemental UV-B of well |
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| Keywords: | One-year-old Panax notoginseng plants Photosynthetic pigment Phenol and total saponin contents Supplemental UV-B Response |
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