| 基于响应曲面法优化蜂胶的乙醇提取工艺 |
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| 引用本文： | 程菲菲,赵军旗,石鹏君,李江,姚斌.基于响应曲面法优化蜂胶的乙醇提取工艺[J].中国农业科技导报,2012,14(1):85-93. |
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| 作者姓名： | 程菲菲 赵军旗 石鹏君 李江 姚斌 |
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| 作者单位： | (中国农业科学院蜜蜂研究所, 农业部授粉昆虫生物学重点开放实验室, 国家蜂产品加工技术研发中心, 北京 100093) |
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| 基金项目： | 农业科技成果转化资金项目(2009GB23260444)资助 |
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| 摘 要： | 蜂胶具有优良的保健效果,一直被作为功能食品而加以应用。为了满足实际生产中提高蜂胶产品质量和生产效率的要求,在充分模拟实际生产条件的基础上,利用响应曲面法对蜂胶提取率和总黄酮含量的影响因素（提取时间、乙醇浓度、固液比、提取次数、提取温度）进行了分析。结果表明,针对本实验中采用的蜂胶毛胶样品,回归模型预测的蜂胶提取率达到38.324 6%,总黄酮含量达到199.776 0 mg/g；最佳提取条件为提取时间24 h,乙醇浓度95%,固液比1∶10,提取次数2次,提取温度为室温。为了达到指导实际生产的目的,试验结论须得到生产车间的实际检验。
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| 关 键 词： | 蜂胶 提取率 总黄酮含量 响应曲面 |
Cloning,Enzymology Characteristics Analysis of a Xylanase Gene from Phialophora sp.P13 |
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| CHENG Fei-fei
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ZHAO Jun-qi
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SHI Peng-jun
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LI Jiang
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YAO Bin.Cloning,Enzymology Characteristics Analysis of a Xylanase Gene from Phialophora sp.P13[J].Journal of Agricultural Science and Technology,2012,14(1):85-93. |
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| Authors: | CHENG Fei-fei ZHAO Jun-qi SHI Peng-jun LI Jiang YAO Bin |
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| Institution: | (Key Laboratory for Insect-Pollinator Biology, Ministry of Agriculture|National R&D Center for Bee Product Processing|;
Institute of Apiculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China) |
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| Abstract: | Xyn11P13,a glycoside hydrolase family 11 xylanse gene,was cloned from Phialophora sp.P13.The full-length gene consists of 705 bp and encodes 234 amino acids,including a putative signal peptide of 19 residues at N-terminus.The mature xylanase was expressed in Pichia pastoris.In shaker flask culture,XYN11P13 was produced with activity of 11.8 U/mL.XYN11P13 showed the optimal activity at pH 5.5,it retained >80.0% of the maximal activity at pH 3.5~7.0,and showed high stability over the pH range 4.0~10.0.The optimum temperature was 65℃,it retained 80% of the maximal activity at 75℃,and was stable at 60℃.The specific activity,K_m and V_max values towards beechwood xylan were 137 U/mg,6.9 mg/mL and 209 μmol/min/mg,respectively.XYN11P13 was strongly resistant to most metal ions.All these favorable properties make XYN11P13 attractive for potential applications in food and animal feed industries. |
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| Keywords: | Phialophora sp P13 Xylanase cloning Pichia pastoris enzymatic properties |
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