| 一株产碱性蛋白酶的酵母菌发酵条件优化 |
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| 引用本文： | 李亚玲,赵玉洁,谢风行,周可,张峰峰.一株产碱性蛋白酶的酵母菌发酵条件优化[J].天津农业科学,2009,15(1):5-9. |
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| 作者姓名： | 李亚玲 赵玉洁 谢风行 周可 张峰峰 |
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| 作者单位： | 天津市农业生物技术研究中心,天津,300192 |
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| 基金项目： | 天津市应用基础研究计划项目，天津市农科院生物中心所长基金 |
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| 摘 要： | 对一株产碱性蛋白酶假丝酵母菌(Candidasp.N9211)的发酵条件进行了优化,研究各种碳源、氮源及无机盐对产酶的影响,应用正交试验优化发酵培养基组成。结果表明:最佳培养基组成为酵母膏20g/L,蛋白胨15g/L,干酪素20g/L,硫酸亚铁0.01g/L。优化后蛋白酶活性提高了27倍(由1.55U/mL提高到41.85U/mL)。摇瓶培养的最佳条件为:初始pH值10.0,接种量1%,发酵温度35℃,最佳培养时间为15h。
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| 关 键 词： | 碱性蛋白酶 酵母菌 产酶条件 |
Optimization of Medium and Cultivation Conditions for Alkaline Protease Production by Candidasp,N9211 |
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| LI Ya-ling,ZHAO Yu-jie,XIE Feng-xing,ZHOU Ke,ZHANG Feng-feng.Optimization of Medium and Cultivation Conditions for Alkaline Protease Production by Candidasp,N9211[J].Tianjin Agricultural Sciences,2009,15(1):5-9. |
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| Authors: | LI Ya-ling ZHAO Yu-jie XIE Feng-xing ZHOU Ke ZHANG Feng-feng |
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| Institution: | (Tianjin Reararch Center of Agriculture Biotechnology, Tianjin 300192, China) |
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| Abstract: | The culture of Candida sp.N9211 were optimized for the production of alkaline protease. The optimum medium （per liter） was consisted of 20 g yeast extract, 15 g peptone, 20 g casein, 0.01 g FeSO4. After optimization of the culture medium, the enzyme production in shake flasks was enhanced from 1.55 U/mL to 41.85 U/mL. The optimum fermentation conditions were determined as follows： initial pH 10.0, inoculation size 1%, temperature 35℃, and cultivation time 15 h. |
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| Keywords: | alkaline protease yeast fermentation conditions |
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