Determination of the wax ester content in olive oils. Improvement in the method proposed by EEC regulation 183/93 |
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| Authors: | Nota G Naviglio D Romano R Sabia V Musso S S Improta C |
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| Institution: | Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, via Università 100, 80055 Portici, Napoli, Italy. |
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| Abstract: | A simpler and faster procedure than the official one described in document IV of European Economic Community Regulation 183/93 is proposed. The wax ester fraction is isolated from triglycerides using a commercially available silica gel column and carbon tetrachloride as eluent. The recovered wax ester fraction, with the addition of a suitable internal standard solution, is analyzed by gas chromatography. A column with a 65% phenyl methyl silicone stationary phase allows a satisfying separation of wax ester fraction in comparison with both a steryl ester and a light fraction eluted before the internal standard. Furthermore, also the single components of the wax ester fraction are suitably separated. |
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