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杏贮藏过程中主要致病菌的分离与鉴定
引用本文:陈存坤,高芙蓉,冯叙桥,王文生,李国华,于晋泽,纪海鹏.杏贮藏过程中主要致病菌的分离与鉴定[J].保鲜与加工,2014,14(6):30-33.
作者姓名:陈存坤  高芙蓉  冯叙桥  王文生  李国华  于晋泽  纪海鹏
作者单位:国家农产品保鲜工程技术研究中心(天津),天津市农产品采后生理与贮藏保鲜重点实验室;沈阳农业大学食品学院;渤海大学化学化工与食品安全学院;国家农产品保鲜工程技术研究中心(天津),天津市农产品采后生理与贮藏保鲜重点实验室;国家农产品保鲜工程技术研究中心(天津),天津市农产品采后生理与贮藏保鲜重点实验室;国家农产品保鲜工程技术研究中心(天津),天津市农产品采后生理与贮藏保鲜重点实验室;国家农产品保鲜工程技术研究中心(天津),天津市农产品采后生理与贮藏保鲜重点实验室
基金项目:“十二五”农村领域国家科技计划课题(2012AA101606-02);2013农业部公益性行业科研专项(201303075); 2013年天津科技创新体系及平台建设计划项目(12TXGCCX00400) ;天津农业科学院院长基金项目(13007);天津农业科学院院长基金项目(14004)
摘    要:为有效控制杏果采后贮藏中的主要致病菌,对贮藏中腐烂小白杏果实上的病菌进行了分离与鉴定。分离并筛选得到了3种主要霉菌,经过形态结构鉴定和显微镜观察,确定为粉红单端孢霉(Trichothecium roseum)、链格孢霉(Alternaria alternata)和天门冬拟茎点霉(Phomopsis asparagi)。经过回接发现,该3种霉菌能使健康杏果致病,且病状与自然发病一致,从这些病斑中又分离得到了相同的霉菌,从而确定该3种霉菌为小白杏采后的主要致病菌。

关 键 词:  病原菌  粉红单端孢霉  链格孢霉  天门冬拟茎点霉

Isolation and Identification of the Main Pathogenic Fungus from Apricot during Storage
Institution:CHEN Cun-kun, GAO Fu-rong, FENG Xu-qiao, WANG Wen-sheng, LI Guo-hua, YU Jin-ze, JI Hai-peng ( 1. National Engineering and Technology Research Center for Preservation of Agricultural Products(Tianjin), Tianjin Key Laboratory of Physiology and Storage of Agricultural Products Ministry, Tianjin 300384, China; 2. College of Food, Shenyang Agriculture University, Shenyang 110866, China; 3. Food Safety Key Lab of Liaoning Province, Food Science Research Institute of Bohai University, Jinzhou 121013, China)
Abstract:In order to effectively control the main pathogens of postharvest apricot fruit during storage, the major pathogens from rotting Xiaobai apricot fruit were separated and identified. Trichothecium roseum, Alternaria alternata and Phomopsis asparagi were separated and screened by morphological and microscopic observation. After the back inoculation experiment, the three kinds of fungi all could make healthy apricot pathogenic, the symptoms were consistent with the natural occurrence of symptoms, and then we got the same mold from these disease spots, so the determined three kinds of mildews were the major pathogens of postharvest Xiaobai apricot fruit.
Keywords:apricot  pathogens  Trichothecium roseum  Alternaria alternata  Phomopsis asparagi
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