Assessment of early blight (<Emphasis Type="Italic">Alternaria solani</Emphasis>) resistance in tomato using a droplet inoculation method |
| |
| Authors: | Reni Chaerani Remmelt Groenwold Piet Stam Roeland E Voorrips |
| |
| Institution: | (1) Plant Research International, PO Box 16, Wageningen, 6700, AA, The Netherlands;(2) Laboratory of Plant Breeding, Department of Plant Sciences, Wageningen University, Wageningen, The Netherlands;(3) Present address: Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jln. Tentara Pelajar no. 3A, Bogor, 16111, Indonesia |
| |
| Abstract: | A droplet inoculation method was used for evaluation of tomato resistance to early blight, a destructive foliar disease of
tomato caused by Alternaria solani (Ellis and Martin) Sorauer. In this test method, leaflets are inoculated with small droplets of a spore suspension in either
water or a 0.1% agar solution. Early blight resistance was evaluated based on lesion size. The droplet method better discriminated
the level of resistance (P < 0.001) for a range of spore densities in comparison with the more commonly used spray inoculation method. Lesions generated
by droplet inoculation at 7 days after inoculation ranged from small flecks to almost complete blight with an exponential-like
distribution of lesion sizes. Significant correlations (r = 0.52, 0.58, and 0.63, P < 0.001) were observed across three glasshouse tests of 54 accessions including wild species using the droplet method. The
most resistant accessions included wild species: one accession of Solanum arcanum, three accessions of Solanum peruvianum, one accession of Solanum neorickii, and one of Solanum chilense. Solanum pennellii and Solanum pimpinellifolium accessions were susceptible, whereas Solanum habrochaites and Solanum lycopersicum accessions ranged from susceptible to moderately resistant. The droplet test method is simple to apply, offers a fine discrimination
of early blight resistance levels, and allows objective evaluation. |
| |
| Keywords: | Early blight Alternaria solani Tomato Resistance Screening method |
| 本文献已被 SpringerLink 等数据库收录！ |
|
