| 肺形侧耳交配型研究 |
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| 引用本文： | 李维焕,吴璇,王建彬,冯路瑶,陈蒙蒙,程显好.肺形侧耳交配型研究[J].食用菌学报,2016(3):30-32. |
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| 作者姓名： | 李维焕 吴璇 王建彬 冯路瑶 陈蒙蒙 程显好 |
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| 作者单位： | 鲁东大学农学院,山东省食用菌技术重点实验室,山东烟台264025 |
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| 基金项目： | 国家自然科学基金(31400447)，山东省现代农业产业技术体系食用菌创新团队遗传育种岗位[鲁农科技字(2016)18号]资助 |
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| 摘 要： | 以肺形侧耳(Pleurotus pulmonarius)鲁大秀珍1号(LD1)的新鲜子实体为材料,收集担孢子并分离获得58株单核菌株,经过三轮交配实验,将58株单核菌株分成4组(T1、T2、T.和T4),分别有16、14、12和16株,可知肺形侧耳属于四极性异宗配合;指定T1为A1B1,T2为A2B2,核迁移实验明确了T3的交配型为A1B2,T4为A2B1;卡方检验结果表明,4种交配型单核体的比例符合1∶1∶1∶1的分离规律.
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| 关 键 词： | 肺形侧耳 交配型 三轮交配 四极性异宗配合 核迁移实验 |
Mating Type Factors in Pleurotus pulmonarius |
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| Abstract: | A total of 58 Pleurotus pulmonarius monokaryons,identified by the absence of clamp connections,were obtained by single-spore separation from a fresh fruit body (LD1).These were separated into four groups (T1,T2,T3 and T4) containing 16,14,12 and 16 monokaryons respectively.We propose that the P.pulmonarius mating system is tetrapolar heterothallic,and data derived from mating and nuclear transfer experiments indicated the mating loci combinations of T1,T2,T3 and T4 were A1B1,A2B2,A1B2 and A2B1,respectively.Chi-square testing revealed the ratio of the different mating types to be 1∶1∶1∶1. |
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| Keywords: | Pleurotus pulmonarius mating type three-cycle mating reaction tetrapolar heterothallism nuclear migration test |
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