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三种因素对“沪农灵芝1号”子实体三萜的影响
引用本文:刘艳芳,吕佳维,冯娜,张劲松,王晨光,杨焱,张忠,吴迪,周帅,唐传红,唐庆九.三种因素对“沪农灵芝1号”子实体三萜的影响[J].食用菌学报,2016(3):43-48.
作者姓名:刘艳芳  吕佳维  冯娜  张劲松  王晨光  杨焱  张忠  吴迪  周帅  唐传红  唐庆九
作者单位:上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验室,上海201403
基金项目:农业部公益性行业(农业)科研专项(201303080)
摘    要:以8种灵芝三萜标准品为对照,考察栽培生境、栽培方式、采收时期三种因素对我国灵芝主栽品种“沪农灵芝1号”子实体中三萜含量和指纹图谱的影响.结果表明,三种因素对灵芝子实体中的三萜含量具有显著影响,福建栽培所得灵芝子实体中三萜含量较高,代料栽培子实体三萜含量显著高于段木栽培,成熟期灵芝子实体的三萜含量较高;随着孢子粉采收时间的延长,子实体中三萜含量逐渐降低,但是各因素对灵芝子实体中三萜指纹图谱信息无显著影响,相似度均在0.93以上.

关 键 词:灵芝  三萜  指纹图谱  高效液相色谱

Effects of Cultivation Habitat,Cultivation Method and Maturation Stage on the Triterpene Content and Fingerprint of Ganoderma lucidum Cultivar "Hunonglingzhi 1" Fruit Bodies
Abstract:Cultivation habitat,cultivation method and maturation stage all significantly affected the levels of eight triterpenes in fruit bodies of Ganoderma lucidurn cultivar "Hunonglingzhi 1".Fruit bodies cultivated at Taining in Fujian Province contained the highest triterpene levels compared with four other cultivation locations in Jilin,Zhejiang and Shandong Provinces and Shanghai Municipality,and bag-grown fruit bodies were richer in triterpenes compared with log cultivated mushrooms.Triterpene levels were also higher in fruit bodies harvested at the mature stage (log cultivation six months,bag cultivation 2-3 months,after inoculation) compared with 40-day and 80-day post maturity stages,with triterpene levels gradually decreasing over time.Triterpene fingerprints were unaffected by any of the three test parameters.
Keywords:Ganoderma lucidum  triterpene  fingerprint  HPLC
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