| MADS-box基因GmAGL15在大豆种子发育过程中的表达 |
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| 引用本文： | 汪潇琳,陈艳萍,喻德跃.MADS-box基因GmAGL15在大豆种子发育过程中的表达[J].作物学报,2008,34(2):330-332. |
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| 作者姓名： | 汪潇琳 陈艳萍 喻德跃 |
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| 作者单位： | 南京农业大学国家大豆改良中心/作物遗传与种质创新国家重点实验室, 江苏南京210095 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)
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国家高技术研究发展计划(863计划) |
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| 摘 要： | 以同源序列法克隆了大豆MADS-box基因GmAGL15, 序列分析表明, 该基因编码的氨基酸与拟南芥AGL15蛋白的同源性为61%, 其中在MADS区的同源性为84.3%, K区的同源性为63.2%。半定量RT-PCR分析表明, GmAGL15在幼胚中高度表达, 而没有检测到在根、茎、叶和花中的表达。荧光定量RT-PCR分析发现, GmAGL15在大豆种子发育的不同时期均有不同程度的表达, 其中在开花后15 d的幼胚中表达量较高, 而在开花后30 d的幼胚中表达量最高。以上结果显示, 基因GmAGL15对于大豆种子发育的调控可能有重要作用。
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| 关 键 词： | 大豆 种子发育 GmAGL15 表达 |
| 收稿时间： | 2007-05-23 |
| 修稿时间： | 2007-08-19 |
Expression of the MADS-Box Gene GmAGL15 in Seed Development of Soybean |
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| WANG Xiao-Lin,CHEN Yan-Ping,YU De-Yue.Expression of the MADS-Box Gene GmAGL15 in Seed Development of Soybean[J].Acta Agronomica Sinica,2008,34(2):330-332. |
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| Authors: | WANG Xiao-Lin CHEN Yan-Ping YU De-Yue |
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| Institution: | National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China |
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| Abstract: | AGL15 (AGAMOUS-like 15) is the only MADS domain regulatory factor identified to date that is expressed in a highly preferential manner in developing plant embryos. In this study, an AGL15–like gene was obtained from soybean by homology-based cloning method, here named as GmAGL15. Sequence analysis indicated that Arabidopsis AGL15 and GmAGL15 shared 61% of identity at amino acid level, especially 84.3% in the MADS domains, 63.2% in the K domains. RT-PCR analysis showed that GmAGL15 expressed only in embryos. A real-time PCR was performed to observe the expression profiling of GmAGL15 in developing seeds, and the results indicated that the expression of GmAGL15 appeared two peak values, at 15 DAF (days after flowering) and at 30 DAF. GmAGL15 was expressed at approximately 10-fold higher levels at the 30 DAF than other time of seed development. Based on these data, we suggest that GmAGL15 is likely to be an important component of the regulatory circuitry directing seed specific processes in soybean. |
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| Keywords: | Soybean (Glycine max L ) Seed development GmAGL15 Expression |
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