| 大珠母贝金属硫蛋白cDNA克隆与序列特征分析 |
| |
| 引用本文： | 唐仁生,夏建红,王玉梅,龚世园,喻达辉.大珠母贝金属硫蛋白cDNA克隆与序列特征分析[J].安徽农业科学,2009,37(7):2888-2890. |
| |
| 作者姓名： | 唐仁生 夏建红 王玉梅 龚世园 喻达辉 |
| |
| 作者单位： | 华中农业大学水产学院,湖北武汉,430070;中国水产科学研究院南海水产研究所,广东广州,510300;中国水产科学研究院南海水产研究所,广东广州,510300;中国水产科学研究院南海水产研究所,广东广州,510300;上海海洋大学,上海,201303;华中农业大学水产学院,湖北武汉,430070 |
| |
| 基金项目： | 国家高技术研究发展计划863项目(2006AA10A415); 广东省科技兴海项目(A200701C02); 中央级公益性科研院所基本科研业务费专项资金项目(2007TS07) |
| |
| 摘 要： | 目的]探讨重金属对大珠母贝苗种存活的影响。方法]对大珠母贝金属硫蛋白基因进行克隆和序列分析。结果]结果表明,通过构建大珠母贝外套膜全长cDNA文库,首次克隆得到大珠母贝金属硫蛋白（PmMT）全长cDNA序列。该序列全长599 bp,5′UTR（Un-translated region）为75 bp,3′UTR为296 bp,开放阅读框（Open reading frame,ORF）为228 bp,编码75个氨基酸。编码的氨基酸中半胱氨酸含量丰富,达29.3%;赖氨酸和甘氨酸含量也较高,均为9.3%;不含苯丙氨酸、组氨酸、色氨酸和酪氨酸等芳香族氨基酸;含有软体动物等无脊椎动物金属硫蛋白的特征序列。序列特征分析表明,该序列具备金属硫蛋白的典型特征,是金属硫蛋白家族成员。结论]该研究为海洋贝类金属硫蛋白的进化及功能研究提供基础资料。
|
| 关 键 词： | 大珠母贝 金属硫蛋白 cDNA文库 |
Analysis on Cloning and Sequence Characteristics of Metallothionein cDNA in Pinctada maxima |
| |
| TANG Ren-sheng et al.Analysis on Cloning and Sequence Characteristics of Metallothionein cDNA in Pinctada maxima[J].Journal of Anhui Agricultural Sciences,2009,37(7):2888-2890. |
| |
| Authors: | TANG Ren-sheng |
| |
| Institution: | TANG Ren-sheng et al(College of Fisheries,Huazhong Agricultural University,Wuhan,Hubei 430070) |
| |
| Abstract: | Objective]The purpose of this research was to discuss the effect of heavy metal on fingerlings survival of Pinctada maxima.Method]The metallothionein gene of Pinctada maxima was cloned,sequenced and characterized through construction and screening of the full-length cDNA library with tissue from the mantle of the oyster.Result] The results indicated that the full-length cDNA of PmMT was 599 bp,including a 5′ untranslated region(UTR) of 75 bp,a 3′ UTR of 296 bp and an open reading frame(ORF) of 228 bp encoding a polypeptide of 75 amino acids.The contents of cys,lys and gly in the PmMT amino acid sequence were 29.3%,9.3% and 9.3%,respectively,while no phe,his,trp or tyr was found.PmMT was characterized by the conserved features of invertebrate′s MTs,indicating that PmMT was a member of the metallothionein protein family.Conclusion]This research will provide basic information for evolution and function study of marine shellshef metallothionein. |
| |
| Keywords: | Pinctada maxima Metallothionein cDNA library |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
