| 生防链霉菌JH108-2的发酵培养基优化 |
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| 引用本文： | 刘亮山,林茂松,鄢小宁,丁晓帆.生防链霉菌JH108-2的发酵培养基优化[J].植物病理学报,2006,36(3):259-266. |
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| 作者姓名： | 刘亮山 林茂松 鄢小宁 丁晓帆 |
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| 作者单位： | 1. 南京农业大学植物保护学院, 南京 210095;2. 中国热带农业科学院环境与植物保护研究所, 儋州 571737 |
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| 基金项目： | 江苏省南京市科技局科研项目 |
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| 摘 要： | 为了提高链霉菌菌株JH108-2的发酵滤液对植物寄生线虫的毒力,采用正交试验设计,优化其发酵培养基。使用优化培养基大豆粉20g、(NH4)2SO4 6g、蛋白胨3g、葡萄糖40g、酵母粉6g,NaCl 2g、K2HPO4 0.6g.CaCO3 2g、蒸馏水1000mL、pH 6.0]所得的发酵滤液,稀释10倍,处理南方根结线虫(Meloidogyne incognita)二龄幼虫,24h后校正死亡率达95.4%,比对照培养基的发酵滤液所产生的校正死亡率高6.9%,差异达到极显著水平(P=0.01)。
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| 关 键 词： | 链霉菌 正交设计 发酵培养基 南方根结线虫 |
| 文章编号： | 0412-0914(2006)03-0259-08 |
| 修稿时间： | 2004年8月2日 |
Optimization of fermentation medium for biocontrol strain Streptomyces spp. JH108-2 to improve the toxicity to nematode |
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| LIU Liang-shan,LIN Mao-song,YAN Xiao-ning,DING Xiao-fan.Optimization of fermentation medium for biocontrol strain Streptomyces spp. JH108-2 to improve the toxicity to nematode[J].Acta Phytopathologica Sinica,2006,36(3):259-266. |
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| Authors: | LIU Liang-shan LIN Mao-song YAN Xiao-ning DING Xiao-fan |
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| Institution: | 1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;2. Environmental and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China |
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| Abstract: | This study aimed at improvement of the fermentation medium of biocontrol strain Streptomyces spp.JH108-2 to achieve better toxic effect of the culture filtrate on plant parasitic nematodes.The fermentation(medium) was modified by using an orthogonal design method and the optimized medium is composed of:(soybean) farina 20 g;(NH_4)_2SO_4 6 g;glucose 40 g;peptone 3 g;yeast extract 6 g;K_2HPO_4 0.6 g;NaCl(2 g);CaCO_3 2 g;and water to 1 000 mL.The pH was adjusted to 6.0.The 10-fold diluted culture filtrate from optimized fermentation medium showed a modified mortality of 95.4% on second-stage juveniles(J2s) of the root-knot nematode Meloidogyne incognita,which is significantly higher compared to the initial me-(dium). |
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| Keywords: | Streptomces spp orthogonal design fermentation medium Meloidogyne incognita |
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