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| 碱性磷酸酯酶基因在鸡胚和人乳腺癌细胞中的表达 | |
| 引用本文： | 刘影,陈立侨,黄伟达.碱性磷酸酯酶基因在鸡胚和人乳腺癌细胞中的表达[J].中国家禽,2007,29(15):17-19. |
| 作者姓名： | 刘影 陈立侨 黄伟达 |
| 作者单位： | 1. 上海农林职业技术学院,上海,201600 2. 华东师范大学生命科学学院,上海,200062 3. 复旦大学生命科学学院,上海,200433 |
| 摘 要： | 通过PCR方法从pSEAP2-control质粒获得人胎盘碱性磷酸酯酶(PLAP)基因,并克隆到鸡输卵管特异性表达载体(pAB-Sig-SV40)卵清蛋白基因调控区下游.pSEAP2-contro1和pAB-Sig-AP-SV40质粒转染鸡胚细胞和乳腺癌细胞(MCF-7),转染48h后以对硝基苯磷酸钠(pNPP)为底物检测PLAP的活性,结果pSEAP2-contro1在鸡胚细胞和MCF-7细胞中表达PLAP,pAB-Sig-AP-SV40在鸡胚细胞中不表达,而在MCF-7细胞中表达.说明鸡胚细胞可以表达有活性的人源糖蛋白,另外也说明在雌激素受体细胞中,鸡卵清蛋白基因启动子可启动外源基因表达. |
| 关 键 词： | 卵清蛋白基因启动子 鸡胚细胞 MCF-7细胞 表达 |
| 修稿时间： | 2007年4月29日 |
Expression of Human Placental Alkaline Phosphatase Gene in Chicken Embryo Cells and Human Breast Cancer Cells |
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| Liu Ying,Chen Liqiao,Huang Weida.Expression of Human Placental Alkaline Phosphatase Gene in Chicken Embryo Cells and Human Breast Cancer Cells[J].China Poultry,2007,29(15):17-19. | |
| Authors: | Liu Ying Chen Liqiao Huang Weida |
| Abstract: | The coding sequence of human placental alkaline phosphatase(PLAP) gene was amplified from the pSEAP2-control plasmid by polymerase chain reaction(PCR). The plap gene was subcloned into chicken oviduct-specific expression vector(pAB-Sig-SV40). pSEAP2-control and pAB-Sig-AP-SV40 were transfected into chick embryo cells and MCF-7 cells. The results showed that expression of pSEAP2-control was achieved in both cells. That the recombinant vector was expressed in MCF-7 cells,but not in chicken embryo cells. These results indicated that human glycoprotein could be expressed in chicken embryo cells, and it had normal enzymatic activity. In addition,the chicken ovalbumin promoter could drive exogenous gene expression in the cells with estrogen receptor. |
| Keywords: | PLAP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |