| Ca对离体筛选耐盐大豆再生植株的影响 |
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| 引用本文： | 陈云昭,闫国华.Ca对离体筛选耐盐大豆再生植株的影响[J].山西农业大学学报(自然科学版),1995,15(3):217-220. |
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| 作者姓名： | 陈云昭 闫国华 |
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| 作者单位： | 山西农业大学农学系 |
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| 摘 要： | 大豆小复叶外植体在含ＮａＣｌ２５、５０、７５和１００ｍｍｏｌ／Ｌ并加Ｃａ（ＮＯ３）２１０ｍｍｏｌ／Ｌ的培养基中，大豆离体胚在含ＮａＣｌ５０和１００ｍｍｏｌ／Ｌ并分别加Ｃａ（ＮＯ３）２、１０、１５和２０ｍｍｏｌ／Ｌ的培养基中进行耐盐筛选培养。实验结果表明，在盐胁迫下加Ｃａ有缓解盐害的效应．小复叶外植体在较高浓度盐胁迫下的出愈率和根、芽分化率增高；对离体胚在盐胁迫下的生长有促进作用，ＮａＣｌ５０盐胁迫下以Ｃａ１５效果最佳。ＮａＣｌ１００盐胁迫下，以Ｃａ２０效果较好．在盐胁迫下加Ｃａ可减少离体胚再生植株中Ｎａ＋和Ｃｌ－的积累，而增加Ｃａ＋和Ｋ＋的积累，并使ＭＤＡ含量减少．因而有减轻Ｎａ＋和Ｃｌ－毒害和膜脂过氧化的作用。
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| 关 键 词： | Ca（NO_3）_2 大豆 盐胁迫 离体筛选 离子积累 MDA |
EFFECT OF Ca ON THE SCREENING OF NaCl-TOLERANT SOYBEANPLANTLET REGENERATED FROM TISSUE CULTURE |
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| Chen Yunzhao et al..EFFECT OF Ca ON THE SCREENING OF NaCl-TOLERANT SOYBEANPLANTLET REGENERATED FROM TISSUE CULTURE[J].Journal of Shanxi Agricultural University,1995,15(3):217-220. |
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| Authors: | Chen Yunzhao |
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| Abstract: | Soybean young compound leaves as explants were cultured on themedia containing 20, 50, 75 and 100 mmol/L NaCl with vitrol were cultured on themedia containing 50 and 100 mmol/L NaCl with 10,15 and 20 mmol/L Ca (NO3)2respectively for screening salt-tolerant plantlets. The results showed that Ca had an effect on relieving salt injury of soybean under salt stress. Under the higher salt stress, Ca increased the callus forming rate and differentiation rate of root and bud of the compound leaf explants. It also promoted the growth of embryo in vitro under salt stress, with Ca 15 is the best diminished the accumulation of Na and Cl- increased that of Ca and K , diminished MDA content of plantlets cultured from embryo in vitro. It indicated that Ca has an effect on relieving injury of Na and Cl-, and relieving membrane lipids superoxidation. |
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| Keywords: | Ca(NO_3)_2 Soybean Salt stress Screening plantlets in vitro Erron accumulation MDA |
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