| 苹果周期蛋白依赖性蛋白激酶亚基基因MdCKS表达载体的构建 |
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| 引用本文： | 焦其庆,陈学森,郑巧娜,崔美,陈晓流.苹果周期蛋白依赖性蛋白激酶亚基基因MdCKS表达载体的构建[J].山东农业科学,2011(8):1-3. |
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| 作者姓名： | 焦其庆 陈学森 郑巧娜 崔美 陈晓流 |
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| 作者单位： | 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安271018 |
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| 基金项目： | 山东省自然科学基金项目(Y2007D26)资助 |
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| 摘 要： | 根据GenBank上与周期蛋白依赖性蛋白激酶基因相关序列，从苹果MdCKS基因编码区设计带有XbaI和SalI酶切位点的引物。以富士MdCKS基因的eDNA为模板将PCR扩增片段连接到pMD18-TSim-pieVector上，测序正确后，再连接到植物表达载体pBII21上，经过抗性筛选和测序验证，成功构建了MdCKS基因表达载体。并通过电击转化法导入农杆菌LBA4404，以备将来用于番茄转化。
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| 关 键 词： | 苹果 MdCKS 表达载体 |
Construction of Plant Expression Vector of Apple MdCKS Gene |
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| JIAO Qi-qing,CHEN Xue-sen,ZHENG Qiao-na,CUI Mei,CHEN Xiao-liu.Construction of Plant Expression Vector of Apple MdCKS Gene[J].Shandong Agricultural Sciences,2011(8):1-3. |
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| Authors: | JIAO Qi-qing CHEN Xue-sen ZHENG Qiao-na CUI Mei CHEN Xiao-liu |
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| Institution: | JIAO Qi-qing,CHEN Xue-sen,ZHENG Qiao-na,CUI Mei,CHEN Xiao-liu(College of Horticulture Science and Engineering of Shandong Agricultural University/ State Key Laboratory of Crop Biology,Taian 271018,China) |
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| Abstract: | Based on the sequences of the cyclin-dependent kinase genes in GenBank,the primers were designed for PCR with the restriction enzyme sites of XbaⅠ and SalⅠfrom the ORF of the MdCKS gene for PCR.The template was the cDNA of MdCKS from Fuji apple.The PCR fragment was firstly inserted into the pMD18-T Simple Vector,and then inserted into the plant expression vector pBI121 after sequenced.The plant expression vector of MdCKS gene was constructed successfully by resistance selection and sequence confirmation.The recombinant plasmid was transformed into Agrobacterium tumfaciens LBA4404 by electroporation for genetic transformation of tomato. |
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| Keywords: | Apple MdCKS gene Expressing vector |
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