| 酸碱度对气相色谱-质谱联用法检测氯霉素提取的影响 |
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| 引用本文： | 王峰恩,陈子雷,王文博,丁蕊艳,杜红霞,霍鲁格.酸碱度对气相色谱-质谱联用法检测氯霉素提取的影响[J].山东农业科学,2011(7):102-104,106. |
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| 作者姓名： | 王峰恩 陈子雷 王文博 丁蕊艳 杜红霞 霍鲁格 |
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| 作者单位： | 1. 山东省农业科学院中心实验室,山东济南,250100
2. 山东大学化学化工学院,山东济南,250100 |
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| 摘 要： | 为使检测结果更准确，研究了溶液酸碱度对氯霉素提取的影响。在9种不同pH值的水溶液中添加氯霉素标准品，乙酸乙酯提取后，用N，O-双（三甲基硅基）三氟乙酰胺（BSTFA）-三甲基氯硅烷（TMCS）进行硅烷化处理，用正己烷溶解后，使用气相色谱-质谱联用法（GC／MS）检测氯霉素衍生物。通过全扫描质谱图和NIST标准质谱图的对比，对氯霉素衍生物进行定性确定；通过测定特征离子m／z225的值对其定量；再将提取后的水溶液残液全部调至pH=5．5左右，重新检测以确认氯霉素在一些样品中未检出的原因。结果表明，强酸强碱中的氯霉素发生不可逆的分解，在提取氯霉素时应保持中性偏酸环境以达到最佳提取效果。
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| 关 键 词： | 酸碱度 气相色谱-质谱 氯霉素 提取 |
Effect of Solution pH Value on Chloramphenicol Extraction Detected by Gas Chromatography-Mass Spectrometry |
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| WANG Feng-en,CHEN Zi-lei,WANG Wen-bo,DING Rui-yan,DU Hong-xia,HUO Lu-ge.Effect of Solution pH Value on Chloramphenicol Extraction Detected by Gas Chromatography-Mass Spectrometry[J].Shandong Agricultural Sciences,2011(7):102-104,106. |
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| Authors: | WANG Feng-en CHEN Zi-lei WANG Wen-bo DING Rui-yan DU Hong-xia HUO Lu-ge |
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| Institution: | WANG Feng-en1,CHEN Zi-lei1,WANG Wen-bo1,DING Rui-yan1,DU Hong-xia1,HUO Lu-ge2(1.Central Laboratory of Shandong Academy of Agricultural Sciences,Jinan 250100,China,2.College of Chemistry and Chemical Engineering,Shandong University,China) |
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| Abstract: | To make sure the results more accurate,the effects of different solution pH values on the chloramphenicol extraction were studied.The standard chloramphenicol was added into 9 kinds of solutions with different pH values,and then extracted with ethyl acetate after 24 hours.After silylation processing with N,O-di(trimethyl silicon-based) three fluorine acetamide(BSTFA)-trimethyl chlorine silicon hydride(TMCS) and dissolved by normal hexane,the chloramphenicol derivatives were detected by gas chromatography-ma... |
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| Keywords: | pH Gas chromatography-mass spectrometry(GC/MS) Chloramphenicol Extraction |
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