| 超高效液相色谱-串联质谱法测定葡萄中16种植物生长调节剂的残留量 |
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| 引用本文： | 占绣萍,陈建波,马琳,陈秀,黄兰淇,刘彬.超高效液相色谱-串联质谱法测定葡萄中16种植物生长调节剂的残留量[J].农药科学与管理,2020,41(4):32-45. |
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| 作者姓名： | 占绣萍 陈建波 马琳 陈秀 黄兰淇 刘彬 |
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| 作者单位： | 上海市农业技术推广服务中心,农业农村部农药质量监督检验测试中心(上海),上海201103;上海市青浦区蔬菜技术推广站,上海201200 |
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| 基金项目： | 上海市农业农村委农产品风险监测项目 |
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| 摘 要： | 建立了一种用高效液相色谱-串联质谱同时快速检测葡萄中16种植物生长调节剂残留的分析方法。样品采用含5%乙酸的乙腈溶液提取,用盐包脱水,分散固相萃取剂方法净化,采用电喷雾(ESI)正负离子切换模式电离,多反应监测(MRM)模式检测,标准曲线外标法定量。表明,16种农药在0.01~0.5mg/kg的浓度范围内线性良好(r≥0.9932),在三档0.05、0.1、0.5mg/kg添加浓度下,平均回收率为71%~114.5%,相对标准偏差(RSD)在0.9%~17.8%之间,方法的定量限(LOQ)均为0.05mg/kg。该方法简便、快速、灵敏、准确,满足目前国内外对植物生长调节剂残留限量的要求,可为葡萄中植物生长调节剂的监管提供技术支撑。
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| 关 键 词： | 超高效液相色谱-串联质谱 植物生长调节剂 葡萄 残留 |
Determination of 16 Plant Growth Regulator Residues in Grapes by High Performance Liquid Chromatography-tandem Mass Spectrometry |
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| Zhan Xiuping,Chen Jianbo,Ma Lin,Chen Xiu,Huang Lanqi,Liu Bin.Determination of 16 Plant Growth Regulator Residues in Grapes by High Performance Liquid Chromatography-tandem Mass Spectrometry[J].Pesticide Science and Administration,2020,41(4):32-45. |
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| Authors: | Zhan Xiuping Chen Jianbo Ma Lin Chen Xiu Huang Lanqi Liu Bin |
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| Institution: | (Shanghai Agriculture Technology Extension&Service Centre,Pesticide Quality Supervision And Inspection Testing Centre(Shanghai),Ministry of Agriculture and Rural Affairs,Shanghai 201103,China;Vegetable Technical Extension Station Qingpu District Shanghai,Shanghai 201200,China) |
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| Abstract: | An ultra-high performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS)method was established for the determination of 16 plant growth regulator residues in grapes.The grape samples were extracted with acetonitrile containing 1%acetic acid,and dehydrated with a mixture of buffer salts,and purified by dispersive solid phase extraction method.The analysis of 16 plant growth regulators was carried out under electrospray ionization(ESI)mode and multiple reaction monitoring(MRM)mode,quantified by matrix matching curve external standard method.The results showed that the calibration curves of 16 plant growth regulators were linear(r≥0.9932)in the concentration range of 0.01~0.5 mg/kg.The average recoveries at three spiked levels of 0.05,0.1 and 0.5 mg/kg were in the ranges of 71%~114.5%,with relative standard deviations(RSDs)of 0.9%~17.8%.The quantitation limit(LOQ)was 0.05 mg/kg.The method is simple,rapid,sensitive and accurate enough for the confirmation and quantitative determination of multiple pesticide residues in grapes,and is capable of providing technical support for the regulation of plant growth regulator residues in common grapes. |
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| Keywords: | UPLC-MS/MS plant growth regulator grape residue |
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