| 人参水溶性蛋白的纯化工艺研究 |
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| 引用本文： | 张巍,李红艳,马晶,姜先刚,赵雨,赵大庆.人参水溶性蛋白的纯化工艺研究[J].吉林农业大学学报,2008,30(1):36-39. |
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| 作者姓名： | 张巍 李红艳 马晶 姜先刚 赵雨 赵大庆 |
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| 作者单位： | 长春中医药大学中医药与生物工程研发中心,长春,130117 |
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| 基金项目： | 科技部“十五”攻关项目(2004BA907A17) |
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| 摘 要： | 为研究人参中几种水溶性蛋白的提取纯化工艺,采用中性缓冲液抽提和硫酸铵分级沉淀法提取人参总蛋白,应用超滤、亲和层析、离子交换层析、疏水层析、凝胶过滤层析等方法进行分离、纯化,得到5种水溶性人参蛋白。经SDS-PAGE电泳和高效凝胶过滤色谱分析,其分子量分别为65.8,65.5,24.7,15.1,7.6 kD。研究结果表明,本试验所确定的人参水溶性蛋白纯化工艺路线简便、可行。
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| 关 键 词： | 人参 水溶性蛋白 纯化 |
| 文章编号： | 1000-5684(2008)01-0036-04 |
| 修稿时间： | 2007年2月14日 |
Purification of several ginseng Proteins |
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| ZHENG Wei,LI Hong-yan,MA Jing,JIANG Xian-gang,ZHAO Yu,ZHAO Da-qing.Purification of several ginseng Proteins[J].Journal of Jilin Agricultural University,2008,30(1):36-39. |
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| Authors: | ZHENG Wei LI Hong-yan MA Jing JIANG Xian-gang ZHAO Yu ZHAO Da-qing |
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| Abstract: | The purification progress of several ginseng proteins was studied.The hydrophilic proteins of ginseng were purified by means of salt solution extraction,ammonium sulfate fractionation,ultrafiltration, affinity chromatography on Affi-Gel blue gel,ion exchange chromatography on DEAE-Sepharose and SP-Sepharose,hydrophobic interaction chromatography on Phenyl-Sepharose.Five ginseng proteins were obtained.Their purities were analysed by SDS-polyacrylamide gels electrophoresis and their molecular weights were determined to be 65.8,65.5,24.7,15.1,7.6 kD,respectively,by Gel Filtration Chromatography.This method is simple and feasible. |
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| Keywords: | ginseng hydrophilic protein purification |
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