| 水稻白叶枯病菌gacA基因的克隆、测序及敲除研究 |
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| 引用本文： | 杨万风,刘红霞,娄旸,胡白石,许志刚,刘凤权.水稻白叶枯病菌gacA基因的克隆、测序及敲除研究[J].南京农业大学学报,2006,29(3):33-38. |
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| 作者姓名： | 杨万风 刘红霞 娄旸 胡白石 许志刚 刘凤权 |
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| 作者单位： | 南京农业大学农业部病虫监测与治理重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | 根据黄单胞菌gacA基因的同源性设计简并引物，采用PCR方法从水稻白叶枯病菌（Xanthomonas oryzae pvoryzae，Xoo）中克隆了gacA同源基因，命名为gacAxoo。序列比较显示，该基因在黄单胞菌中是相对保守的。蛋白质保守结构域搜索表明，GacAxoo属于LuxR家庭的一员，具有与其他GacA蛋白相似的结构。通过同源重组的方法，构建了gacAxoo的插入突变体，为下一步研究gacAxoo的功能奠定了良好的基础。
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| 关 键 词： | 水稻白叶枯病菌 gacA基因 基因敲除 |
| 文章编号： | 1000-2030(2006)03-0033-06 |
| 收稿时间： | 10 14 2005 12:00AM |
| 修稿时间： | 2005年10月14 |
Cloning, sequencing and knock-out of gacA gene from Xanthomonas oryzae pv. oryzae |
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| YANG Wan-feng,LIU Hong-xia,LOU Yang,HU Bai-shi,XU Zhi-gang,LIU Feng-quan.Cloning, sequencing and knock-out of gacA gene from Xanthomonas oryzae pv. oryzae[J].Journal of Nanjing Agricultural University,2006,29(3):33-38. |
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| Authors: | YANG Wan-feng LIU Hong-xia LOU Yang HU Bai-shi XU Zhi-gang LIU Feng-quan |
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| Institution: | Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | A gacA homologue,designated gacA_Xoo,was cloned from the genomic DNA of Xanthomonas oryzae pv.oryzae(Xoo),a bacterium that causes bacterial blight of rice,with degenerated primers by polymerase amplification reaction(PCR).NCBI blast search indicated that GacA_Xoo had a similar structure to that of other GacA proteins,and had conserved domain of LuxR-like proteins.Sequence comparison showed that gacA_Xoo was conserved in the Xanthomonas genus.A gacA_Xoo disruption mutant was successfully generated by a single cross-over event,selected by Amp resistance on NA medium,and confirmed by PCR and Southern blot.This mutant is a useful tool for further study on the function of gacA_Xoo in X.oryzae pv.oryzae. |
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| Keywords: | Xanthomonas oryzae pv oryzae(Xoo) gacA gene gene knock-out |
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