| 抗中华绒螯蟹维氏气单胞菌中草药的筛选及其复方制剂的配伍优化 |
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| 引用本文： | 曹海鹏,周慧华,顾颖,陈百尧,安健.抗中华绒螯蟹维氏气单胞菌中草药的筛选及其复方制剂的配伍优化[J].上海海洋大学学报,2022,31(6):1413-1422. |
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| 作者姓名： | 曹海鹏 周慧华 顾颖 陈百尧 安健 |
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| 作者单位： | 上海海洋大学 国家水生动物病原库,上海海洋大学 国家水生动物病原库,上海海洋大学 国家水生动物病原库,连云港市海洋与渔业发展促进中心,连云港市海洋与渔业发展促进中心 |
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| 基金项目： | 国家虾蟹产业技术体系项目(CARS-48)；江苏省海洋与渔业科技创新与推广项目(Y2018-8). |
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| 摘 要： | 为研制抗中华绒螯蟹维氏气单胞菌中草药复方制剂,本实验以中华绒螯蟹致病性维氏气单胞菌HXH1为筛选指示菌,在采用试管二倍稀释法筛选出具有良好抑菌活性的抗菌中草药的基础上,采用正交t值法对各抗菌中草药进行了配伍优化制备中草药复方制剂,并进一步通过试管二倍稀释法对中草药复方制剂的体外抗菌效果进行了评价。结果表明,黄连、苏木、大黄、乌梅、鸡血藤、石榴皮、香薷等7种中草药提取液对维氏气单胞菌的抑菌效果较好,对维氏气单胞菌HXH1的最小抑菌浓度范围为7.81-31.25 mg/mL,但因大黄、鸡血藤、乌梅存在负效应,优选黄连、香薷、苏木、石榴皮作为中草药复方制剂的主要成分,其最佳配伍比例为5:4:1:2,在该配伍比例下对维氏气单胞菌HXH1的最小抑菌浓度为5.21 mg/mL。此外,中草药复方制剂对HXY1、HXY3、J1Y4、J1Y5、J2Y4、J2Y6、WAB1等其他中华绒螯蟹源维氏气单胞菌菌株的最小抑菌浓度为3.91-7.81 mg/mL,具有良好的体外抗菌效果。本实验确定了抗中华绒螯蟹维氏气单胞菌中草药复方制剂的主要成分和配伍比例,为维氏气单胞菌引起中华绒螯蟹病害的中草药控制奠定了基础。
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| 关 键 词： | 中草药 维氏气单胞菌 配伍 中华绒螯蟹 |
| 收稿时间： | 2021/12/3 0:00:00 |
| 修稿时间： | 2022/1/14 0:00:00 |
Screening and compatibility optimization of herbs against Aeromonas veronii pathogenic to Eriocheir sinensis |
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| CAO Haipeng,ZHOU Huihu,GU Ying,CHEN Baiyao,AN Jian.Screening and compatibility optimization of herbs against Aeromonas veronii pathogenic to Eriocheir sinensis[J].Journal of Shanghai Ocean University,2022,31(6):1413-1422. |
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| Authors: | CAO Haipeng ZHOU Huihu GU Ying CHEN Baiyao AN Jian |
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| Institution: | National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Lianyungang Marine and Fisheries Development Promotion Center,Lianyungang Jiangsu,Lianyungang Marine and Fisheries Development Promotion Center,Lianyungang Jiangsu |
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| Abstract: | In order to develop herb compound preparation against Aeromonas veronii pathogenic to Eriocheir sinensis, the present study used A. veronii HXH1 as the indicator strain to screen herbs with good inhibitory activities by tube dilution method, and on this basis, the compatibility of these herbs was optimized by orthogonal t design to prepare Chinese herbal compound preparation, and the in vitro antibacterial effect of the Chinese herbal compound preparation was further evaluated using tube dilution method. The results indicated that Rhizoma coptidis, Caesalpinia sappan, Radix et rhizoma rhei, Fructus mume, Spatholobi caulis, Punica granatum, Moslae herba showed highly inhibitory activities against A. veronii, with the minimum inhibitory concentration (MIC) ranging from 7.81 to 31.25 mg/mL against A. veronii HXH1. However, R. et rhizoma rhei, C. sappan and F. mume was found to have negative effects. Thus, R. coptidis, M. herba, C. sappan, P. granatum was chose as main components of the Chinese herbal compound preparation, and the best compatibility proportion was determined as 5:4:1:2, under which the MIC of the compound preparation was 5.21 mg/mL against A. veronii HXH1. In addition, the Chinese herbal compound preparation exhibited good activities against other A. veronii isolates from E. sinensis, showing the MICs of 3.91 to 7.81 mg/mL aginst strains HXY1, HXY3, J1Y4, J1Y5, J2Y4, J2Y6, WAB1. This study determines the main component and compatibility proportion of herb compound preparation against A. veronii pathogenic to E. sinensis, and can provide a basis for the herb control of diseases in E. sinensis. |
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| Keywords: | Herb Aeromonas veronii compatibility Eriocheir sinensis |
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