| 分光光度法测定角果藜中总黄酮含量 |
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| 引用本文： | 刘珊珊,马先梅,杨红兵.分光光度法测定角果藜中总黄酮含量[J].安徽农业科学,2011,39(29):17865-17866. |
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| 作者姓名： | 刘珊珊 马先梅 杨红兵 |
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| 作者单位： | 石河子大学化学化工学院新疆兵团化工绿色过程重点实验室,新疆石河子,832003 |
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| 基金项目： | 新疆兵团国际科技合作计划(2011BC003) |
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| 摘 要： | 目的]建立角果藜中总黄酮含量的测定方法。方法]采用加热回流法提取角果藜中的黄酮类化合物,以芦丁为标准品测定总黄酮含量。结果]采用紫外分光光度法测定总黄酮含量时,在0.019～0.102 mg/ml浓度范围内线性关系良好（r=0.999 1）。方法的精密度和准确度均较高,相对标准偏差为0.226%,平均回收率为99.36%。测得角果藜中总黄酮含量为10.12 mg/g。结论]该方法操作简单,科学准确,适合用于角果藜中总黄酮含量的测定。
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| 关 键 词： | 角果藜 黄酮类化合物 分光光度法 |
Determination of Total Flavones Content in Ceratocarpus arenarius L.by Spectrophotometry |
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| Institution: | LIU Shan-shan et al(Xinjiang Bingtuan Key Laboratory for Green Processing of Chemical Engineering/School of Chemistry and Chemical Engineering,Shihezi University,Shihezi,Xinjiang 832003) |
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| Abstract: | Objective] The aim was to establish the method for determining total flavoens content in Ceratocarpus arenarius L..Method ] Flavonoids were extracted from C.arenarius by Heating refluxing,and determined with rutin as the standard.Result] In the range of 0.019-0.102 mg/ml,linear relationship was good(r=0.999 1).This method had higher precision and accuracy with RSD of 0.226% and recoveries of 99.36%.The total flavoens content from C.arenarius is 10.12 mg/g.Conclusion] The method was simple and reliable,which could be used to control the quality of C.arenariu. |
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| Keywords: | Ceratocarpus arenarius L Flavonoids Spectrophotometry |
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