| GTPase RABE1b参与拟南芥胚胎发育的研究 |
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| 引用本文： | 李元,路小铎,沈颂东,张春义.GTPase RABE1b参与拟南芥胚胎发育的研究[J].安徽农业科学,2011,39(25):15207-15209,15278. |
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| 作者姓名： | 李元 路小铎 沈颂东 张春义 |
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| 作者单位： | 苏州大学基础医学与生物科学学院,江苏苏州215123;中国农业科学院生物技术研究所,北京100081;中国农业科学院生物技术研究所,北京100081;中国科学院植物研究所,北京100093;苏州大学基础医学与生物科学学院,江苏苏州,215123;中国农业科学院生物技术研究所,北京100081;国家农作物基因资源与遗传改良重大科学工程,北京100081 |
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| 摘 要： | 目的]研究影响拟南芥早期胚胎发育分裂模式的基因。方法]从拟南芥T-DNA插入的突变体库中分离到2个T-DNA插入突变体,通过表型观察胚胎发育情况。结果]PCR-WALKING表明,T-DNA插入位点分别位于基因At4g20360的5′非编码区和启动子区,基因编码的GTPase RABE1b蛋白为Rab蛋白家族的成员,将这2个突变体分别命名为Atrabe1b-1和Atrabe1b-2。透明分析发现此突变体在胚胎发育早期球形胚时期分裂模式出现异常,RT-PCR分析发现此基因在拟南芥中以组成型表达。结论]基因At4g20360影响拟南芥早期胚胎发育的分裂模式,推测其编码的蛋白GTPase RABE1b可能对控制拟南芥胚胎发育过程中的细胞分裂起重要作用。
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| 关 键 词： | AtRABE1b 细胞分裂 胚胎发育 GTPase |
Study on the Involvement of GTPase RABE1b in Embryonic Development of Arabidopsis thaliana |
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| Institution: | LI Yuan et al(School of Biology and Basic Medical Sciences,Soochow University,Suzhou,Jiangsu 215123) |
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| Abstract: | Objective] The aim was to study the gene affecting division mode in the early embryonic development of Arabidopsis thaliana.Method] In this study,two T-DNA insertion mutants were isolated from the mutant library of A.thaliana inserted by T-DNA,and the embryonic development was refeleted by phenotypes.Result] Genomic PCR-WALKING analyses demonstrated that T-DNA insertion sites were in 5′ non-coding region and promoter region of gene At4g20360 which encode Rab GTPase RABE1b,and the two mutants were named as Atrabe1b-1 and Atrabe1b-2,respectively.Microscopic analysis on mutants revealed that there was an abnormal cell division pattern in early globular stage of embryonic development.In addition,RT-PCR analysis showed that gene At4g20360 was constitutively expressed in A.thaliana.Conclusion] Gene At4g20360 affected the division pattern of early embryonic development in A.thaliana,and encoded protein GTPase RABE1b which may play an important role in cell division during embryonic development of A.thaliana. |
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| Keywords: | AtRABE1b Cell division Embryonic development GTPase |
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