| 复合酶在叶下珠有效成分提取中的应用 |
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| 引用本文： | 蓝峻峰,廖政达,谢济运,玉澜.复合酶在叶下珠有效成分提取中的应用[J].安徽农业科学,2011,39(25):15254-15255,15257. |
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| 作者姓名： | 蓝峻峰 廖政达 谢济运 玉澜 |
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| 作者单位： | 柳州师范高等专科学校化学与生命科学系,广西柳州,545004;柳州师范高等专科学校化学与生命科学系,广西柳州,545004;柳州师范高等专科学校化学与生命科学系,广西柳州,545004;柳州师范高等专科学校化学与生命科学系,广西柳州,545004 |
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| 摘 要： | 目的]探索复合酶辅助提取叶下珠有效成分的工艺条件,为叶下珠的高效利用提供依据。方法]以水为提取溶剂,以总黄酮提取得率为考察指标,选择料液比、提取温度、提取时间、纤维素酶浓度和果胶酶浓度等5因素4水平,用正交试验优选其最佳工艺条件。结果]复合酶法提取叶下珠有效成分的最佳提取工艺为：提取时间2 h,料液比1∶25（g∶ml）,提取温度40℃,纤维素酶浓度1.4 g/L,果胶酶2.0 g/L。在此条件下,黄酮的提取率为1.589%。试验所考察的5个因素中,对提取率影响的主次顺序为：提取温度〉果胶酶浓度〉料液比〉提取时间〉纤维酶浓度。结论]复合酶法提取省时、高效、低碳耗且提取率高,是一种对环境友好的方法。
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| 关 键 词： | 叶下珠(Phyllanthus urinaria L.) 复合酶 黄酮 提取工艺 |
Complex Enzyme-Assisted Extraction of Effective Components from Phyllanthus urinaria L. |
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| Institution: | LAN Jun-feng et al(Department of Chemistry and Life Science,Liuzhou Teachers College,Liuzhou,Guangxi 545004) |
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| Abstract: | Objective]To optimize the complex enzyme-assisted extraction process of effective components from Phyllanthus urinaria L.,so as to provide references for its high-efficient utilization.Methods]The flavonoids in phyllanthus urinaria L.was extracted by using water as the extraction agent.The extraction conditions were optimized by the orthogonal test with 4 levels and 5 factors,namely,material-liquid ratio,extraction temperature,extraction time,the concentration of cellulose and pectase.Result]The optimum extraction technology was:the extraction time was 2 h,the material-liquid ratio was 1∶ 25,extraction temperature was 40 ℃,the concentration of cellulose was 1.4 g/L and pectase concentration was 2.0 g/L.Under the optimized conditions,the extraction ratio of flavonoids was 1.589%.The influence of the inspected five factors on the extraction yield decreased in the order of extraction temperature>pectase concentration>material-liquid ratio>extraction time>cellulose concentration.Conclusion]Complex enzyme-assisted extraction method was rapid,efficient and environmental-friendly with low carbon consumption and high extraction yield. |
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| Keywords: | Phyllanthus urinaria L Complex enzyme Flavonoids Extraction process |
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