| 海洋硅藻叶绿素a测定方法的改进 |
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| 引用本文： | 施建乐,朱明新,蒋皎梅,徐炎华.海洋硅藻叶绿素a测定方法的改进[J].安徽农业科学,2011,39(23):14233-14234,14252. |
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| 作者姓名： | 施建乐 朱明新 蒋皎梅 徐炎华 |
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| 作者单位： | 南京工业大学环境学院,江苏南京,210009 |
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| 摘 要： | 目的]研究海洋硅藻叶绿素a测定方法的改进。方法]通过对现有的叶绿素a测定方法（规范法、浸泡法、冻融法）进行比较分析,结合硅藻细胞自身特征,对海洋硅藻叶绿素a测定方法进行改进。结果]将原先的冻融法和浸泡法中的手动振荡改为漩涡振荡20m in,并将浸泡法的浸泡时间减少为6 h。对比分析表明,改进后的冻融法和浸泡法的相对标准偏差均低于5%,较原先的冻融法和浸泡法有进一步的降低,且叶绿素a相对值较高。结论]改进后的冻融法和浸泡法更加适合海洋硅藻叶绿素a含量的测定,具有很好的重复性,测量结果相对精确度高,操作简便,适用于数据量较大的试验。
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| 关 键 词： | 硅藻 叶绿素a 浸泡法 冻融法 改进 |
Study on the Improvement of Chlorophyll a Measuring Method in the Marine Diatom |
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| Institution: | SHI Jian-le et al(College of Environment,Nanjing University of Technology,Nanjing,Jiangsu 210009) |
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| Abstract: | Objective]The research aimed to study the improvement of chlorophyll a measuring method in the marine diatom.Method]The existing chlorophyll a measuring methods which included the criterion method,soaking method and freeze-thaw method were contrasted and analyzed.By combining with the self characteristic of diatom cell,the measuring method of chlorophyll a in the marine diatom was improved.Result]The manual oscillation in the original freeze-thaw method and soaking method was changed into the vortex oscillation 20 min.Moreover,the soaking time in the soaking method decreased to 6 h.The contrast and analysis showed that RSD of improved freeze-thaw method and soaking method was lower than 5%,which had the further decrease than that of original freeze-thaw method and soaking method.Moreover,the relative value of chlorophyll a was higher.Conclusion]The improved freeze-thaw method and soaking method were more suitable for the measurement of chlorophyll a content in the marine diatom.They had the good repeatability.The relative accuracy of measured result was high,and the operation was simple.They were suitable for the experiment whose data was large. |
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| Keywords: | Diatom Chlorophyll a Soaking method Freeze-thaw method Improvement |
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