| 基因型及基本培养基对玉米成熟胚培养的影响 |
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| 引用本文： | 吕杰,周晓馥,未晓巍,赵卓,徐洪伟.基因型及基本培养基对玉米成熟胚培养的影响[J].安徽农业科学,2011,39(29):17767-17768,17780. |
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| 作者姓名： | 吕杰 周晓馥 未晓巍 赵卓 徐洪伟 |
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| 作者单位： | 吉林师范大学遗传生理研究所,吉林四平,136000 |
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| 基金项目： | 国家自然科学基金项目“玉米水分、养分资源高效利用根构型的调控机理(31070224)”,国家自然科学基金项目“玉米氮磷高效吸收的根系育种策略及根系发育模型建立(30970219)”;吉林省科技厅项目“超高产、强抗逆及氮磷高效的玉米种质创新(20080203)” |
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| 摘 要： | 目的]筛选出适宜玉米成熟胚组织培养的基因型和基本培养基。方法]以9个基因型的玉米成熟胚为外植体,研究基因型与基本培养基对成熟胚愈伤组织诱导和继代的影响。结果]试验中表现较好的基因型依次是853-35、853-209、丹34和81162;对于玉米成熟胚愈伤组织的诱导培养,MS培养基比N6培养基更适宜,而对于玉米成熟胚愈伤组织的继代培养,N6的培养基比MS培养基更适宜。结论]进一步完善了以玉米成熟胚为外植体的组织培养体系。
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| 关 键 词： | 玉米 成熟胚 基因型 基本培养基 愈伤组织 |
Effects of Genotypes and Basic Medium on Culture of Maize Mature Embryos |
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| Institution: | LV Jie et al(Institute of Genetics and Physiology,Jilin Normal University,Siping,Jilin 136000) |
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| Abstract: | Objective] This study was to screen out suitable genotypes and basic medium for the culture of maize mature embryos.Method] Using mature embryos of nine maize genotypes as explants,the effects of genotypes and basic medium on callus induction and subculture were investigated.Result] The genotypes performed better in callus induction and subculture were found in turn 853-35,853-209,Dan 34 and 81162.MS medium is better than N6 medium in the callus induction from maize embryos,while N6 medium is more suitable for callus subculture.Conclusion] Our study further improved the tissue culture system in maize with mature embryos as explants. |
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| Keywords: | Maize Mature embryos Genotype Basic culture medium Callus induction |
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