| 龙爪槐提取物对铜绿微囊藻生长的抑制作用研究 |
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| 引用本文： | 周晓见,夏洁,靳翠丽,缪莉,董昆明,封克.龙爪槐提取物对铜绿微囊藻生长的抑制作用研究[J].安徽农业科学,2011,39(25):15322-15324. |
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| 作者姓名： | 周晓见 夏洁 靳翠丽 缪莉 董昆明 封克 |
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| 作者单位： | 扬州大学环境科学与工程学院,江苏扬州225127;扬州大学海洋科学与技术研究所,江苏扬州225127;扬州大学环境科学与工程学院,江苏扬州,225127 |
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| 基金项目： | 国家自然科学基金项目，教育部科学技术研宛重点项目，江苏省自然科学基金项目，江苏省环境材料与环境工程重点实验室开放课题，江苏省教育厅研究生科技创新项目，扬州大学“新世纪”人才工程项目 |
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| 摘 要： | 目的]探讨龙爪槐提取物对铜绿微囊藻生长的抑制作用。方法]测定了龙爪槐叶片浸取液对铜绿微囊藻的增殖抑制效果,并对活性组分进行了初步研究和分析。结果]龙爪槐叶片的无水乙醇浸提物经正己烷、乙酸乙酯、正丁醇和水依次分配后,提取物主要成分为极性组分（60%以上）,而含量约为25%的非极性组分（正己烷相）具有显著的抑制铜绿微囊藻生长的效果。在25和50 mg/L正己烷相组分作用下铜绿微囊藻的7 d生长抑制率分别达75%和90%以上,同时还伴随着细胞中叶绿素a的破坏。结论]为防治微囊藻水华提供了理论依据。
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| 关 键 词： | 龙爪槐 铜绿微囊藻 正己烷相 叶绿素a |
Study on the Inhibitory Effect of Sophora japonica Extracts aginst the Growth of Microcystis aeruginosa |
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| Institution: | ZHOU Xiao-jian et al(College of Environmental Science and Engineering,Yangzhou University,Yangzhou,Jiangsu 225127) |
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| Abstract: | Objective] The research aimed to discuss the inhibitory effect of Sophora japonica extracts against the growth of Microcystis aeruginosa.Method] The inhibitory effect of extracted liquid of Sophora japonica leaf against the growth of Microcystis aeruginosa was measured.Moreover,the active component was studied and analyzed initially.Result] The absolute alcohol extract of Sophora japonica leaf was separated to n-hexane,ethyl acetate,n-butanol and water phases in turn.The polar fractions were found being the majority(>60%).The non-polar fraction in n-hexane(about 25%) was found significantly inhibiting the growth of M.aeruginosa.The inhibition rates of fraction in n-hexane at the concentrations of 25 and 50 mg/L against M.aeruginosa in 7 d were higher than 75% and 90% respectively.In addition,chlorophyll a of M.aeruginosa was also destroyed in the presence of the hexane fraction.Conclusion] The research provided the theory basis for preventing and controlling the water bloom of M.aeruginosa. |
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| Keywords: | Sophora japonica Microcystis aeruginosa n-hexane phase Chlorophyll a |
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