| 虾Y-器官7、8-脱氢酶转化胆固醇为7-脱氢胆固醇研究 |
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| 引用本文： | 黄时海,何鑫平,于配配,曹喜秀,曹普美,吴孔阳,李湘萍.虾Y-器官7、8-脱氢酶转化胆固醇为7-脱氢胆固醇研究[J].安徽农业科学,2011,39(25):15397-15399. |
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| 作者姓名： | 黄时海 何鑫平 于配配 曹喜秀 曹普美 吴孔阳 李湘萍 |
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| 作者单位： | 1. 广西大学生命科学与技术学院,广西南宁,530005
2. 广西大学动物繁殖研究所,广西南宁,530005 |
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| 基金项目： | 广西区教委人才项目，国家大学生创新基金，广西大学实验教改项目 |
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| 摘 要： | 目的]以虾Y-器官微粒体7、8-脱氢酶催化胆固醇,制备7-脱氢胆固醇。方法]通过单因素试验,研究影响转化反应的各种因素。结果]将摘除眼柄的刀额新对虾（Metapenaeus ensis）Y-器官,匀浆、离心制备的微粒体酶液,于22℃与胆固醇避光反应6 h,可以积累相对较多的7-脱氢胆固醇。结论]该研究为利用虾Y-器官7、8-脱氢酶转化胆固醇为7-脱氢胆固醇奠定基础。
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| 关 键 词： | 虾Y-器官 7、8-脱氢酶 胆固醇 7-脱氢胆固醇 生物转化 |
Study on the Transformation of Cholesterol to 7-dehydrocholesterol by 7, 8-dehydrogenase from Shrimps' Y-organ |
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| Institution: | HUANG Shi-hai et al(College of Life Science and Technology,Guangxi University,Nanning,Guangxi 530005) |
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| Abstract: | Objective] The research aimed to catalyze cholesterol to prepare 7-dehydrocholesterol by using 7,8-dehydrogenase from shrimps' Y-organ microsome.Method] Using single factor test,various influencing factors of the transformation test were studied.Result] Y organ of Metapenaeus ensis that eyestalk was removed was used to prepare microsomal enzyme solution after homogenation and centrifugation.It was reacted with cholesterin at 22 ℃ under shading conditions,which could accumulate relatively more 7-dehydrocholesterol.Conclusion] The research laid the foundation for transforming cholesterol to 7-dehydrocholesterol using 7,8-dehydrogenase from shrimps' Y-organ. |
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| Keywords: | Shrimps' Y-organ 7 8-dehydrogenase Cholesterol 7-dehydrocholesterol Biotransformation |
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