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Morphology and SSR fingerprinting of newly developed <Emphasis Type="Italic">Cynara cardunculus</Emphasis> genotypes exploitable as ornamentals
Authors:Sergio Lanteri  Ezio Portis  Alberto Acquadro  Rosario P Mauro  Giovanni Mauromicale
Institution:(1) Di.Va.P.R.A. Plant Genetics and Breeding, University of Torino, via L. da Vinci 44, 10095 Grugliasco (Torino), Italy;(2) Dipartimento di Scienze Agronomiche, Agrochimiche e delle Produzioni Animali—sez. Scienze Agronomiche, University of Catania, via Valdisavoia 5, 95123 Cantania, Italy;
Abstract:The species Cynara cardunculus includes the globe artichoke (var. scolymus), the cultivated cardoon (var. altilis) and the wild cardoon (var. sylvestris). The three taxa are sexually compatible and originate fertile F 1 progenies, which, given the high heterozygosity of the species, are highly segregating. We report the characterization of two F 1 populations, one bred from a cross between globe artichoke and cultivated cardoon, and the other between globe artichoke and wild cardoon. Both populations featured a wide array of phenotypes in relation to several traits, and some of the newly developed genotypes are of interest for the ornamental market. The two populations were genotyped at 50 microsatellite (SSR) loci: in the globe artichoke × wild cardoon and globe artichoke × cultivated cardoon progenies 116 and 97 alleles were respectively detected. SSR pattern scores were used to produce an UPGMA dendrogram and a PCoA plot. A set of nine SSR loci, evenly dispersed across the genome, was shown to be sufficient to unambiguously identify each segregant. The molecular fingerprinting is useful for establishing the true to type correspondence of propagative materials in nurseries and ensures the effective correspondence between the real and the declared identity of a clone.
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