| 舟山野菊花序不同萃取物抑菌活性的研究 |
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| 引用本文： | 童国忠,马剑茵,石亚素,王斌.舟山野菊花序不同萃取物抑菌活性的研究[J].浙江水产学院学报,2010(6):562-565. |
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| 作者姓名： | 童国忠 马剑茵 石亚素 王斌 |
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| 作者单位： | [1]浙江海洋学院食品与药学学院,浙江舟山316004 [2]舟山市定海区疾病控制中心,浙江舟山316000 |
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| 基金项目： | 浙江省自然科学基金（Y2080579）; 浙江海洋学院校级重大课题（21135030107） |
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| 摘 要： | 对野菊花序采用微波粗提,经石油醚、乙酸乙酯、正丁醇和水得萃取物,通过K-B法和倍比稀释法,分别对大肠埃希菌、金黄色葡萄球菌的标准、耐药菌株进行体外抑菌活性测试。结果表明：水相的抑菌活性〉正丁醇〉乙酸乙酯,石油醚萃取物无抑菌效果;水相对大肠埃希菌标准、耐药菌株的MIC分别为15.62 mg/mL和7.81 mg/mL,对金黄色葡萄球菌的标准、耐药菌株的MIC分别为3.90 mg/mL和1.95 mg/mL,正丁醇相的MIC在15.62~31.25 mg/mL之间。水相和正丁醇相呈现较强的广谱抑菌活性,且具有抗耐药菌作用。
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| 关 键 词： | 野菊花 萃取 抑菌活性 最低抑菌浓度 |
Antimicrobial Activity of the Extract in the Inflorescence of Chrysanthemum indicum from Zhoushan Archipelago |
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| Institution: | TONG Guo-zhong,MA Jian-yin,SHI Ya-su,et al(1.Food and Pharmacy School of Zhejiang Ocean University,Zhoushan 316004; 2.Dinghai District,Zhoushan Municipal Center for Disease and Control,Zhoushan 316000,China) |
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| Abstract: | The ethanol crude extracts（ECE） in the inflorescence of Chrysanthemum indicum from Zhoushan Archipelago was obtained by microwave assisted extract and further purified by liquid-liquid partition to afford four fractions： petroleum ether（A）,ethyl acetate（B）,butanol（C） and aqueous（D）.Through the K-B method and serial dilution method,the antimicrobial activities of the four fractions on Escherichia coli,Staphylococcus aureus and the standard drug-resistant strains were tested.The order of activities from high to low was fraction D,fraction C,fraction B and fraction A.The MIC of fraction D on Escherichia coli standard and drug-resistant strains,Staphylococcus aureus standard and drug-resistant strains were 15.62 mg/mL,7.81 mg/mL,3.90 mg/mL and 1.95 mg/mL,respectively.The MIC of fraction C on the two standard and drug-resistant strains were between 15.62 mg/mL and 31.25 mg/mL.The data indicated the fraction D and C showed a strong broad-spectrum antibacterial activity and anti-resistant effect. |
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| Keywords: | Chrysanthemum indici extract antimicrobial activity MIC |
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