| 大豆根缘细胞对Al3+毒害的响应 |
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| 引用本文： | 蔡妙珍,刘鹏,徐根娣,刘文秀,龚春风.大豆根缘细胞对Al3+毒害的响应[J].中国农业科学,2007,40(2):271-276. |
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| 作者姓名： | 蔡妙珍 刘鹏 徐根娣 刘文秀 龚春风 |
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| 作者单位： | 浙江师范大学化学与生命科学学院,金华,321004 |
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| 基金项目： | 国家自然科学基金;浙江省自然科学基金 |
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| 摘 要： | 【目的】研究大豆根缘细胞发育的生物学特性和对Al3+毒害的响应，为提高大豆的耐铝性提供理论依据。【方法】以浙春3号和辽鲜3号为材料，采用悬空气培法，研究大豆根缘细胞产生的数目、活性随根长的变化及Al3+毒胁迫下根缘细胞形成和释放的难易程度。【结果】大豆根冠周围新形成的根缘细胞呈圆形，随着生长逐渐发育成椭圆形或矩形或长条形。当根长为15～25 mm时，根缘细胞数目达最大值，浙春3号约3 000个，辽鲜3号约3 800个。去根缘细胞的大豆分别用100、200、300、400 µmol?L-1 Al3+溶液喷洒根尖，根缘细胞的数目和活性明显低于0 µmol?L-1Al3+溶液喷洒的处理，且Al3+浓度越高，根缘细胞数目和活性下降幅度越大。200 µmol?L-1 Al3+、300 µmol?L-1 Al3+处理4 h，两个大豆品种的PME活性明显升高，400 µmol?L-1Al3+处理后PME活性均有明显下降。这说明高浓度Al3+不利于根缘细胞的形成和释放，降低细胞活性和PME活性，辽鲜3号的根缘细胞对Al3+胁迫反应更为敏感。【结论】Al3+毒害时，维持较高根缘细胞的数目、活性及PME活性是抗性品种耐铝毒的重要途径。
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| 关 键 词： | 大豆 根缘细胞 发育 Al3+毒害 |
| 收稿时间： | 2005-9-14 |
| 修稿时间： | 2005-09-22 |
Response of Root Border Cells to Al3+ Toxicity in Soybean |
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| CAI Miao-zhen,LIU Peng,XU Gen-di,LIU Wen-xiu,GONG Chun-feng.Response of Root Border Cells to Al3+ Toxicity in Soybean[J].Scientia Agricultura Sinica,2007,40(2):271-276. |
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| Authors: | CAI Miao-zhen LIU Peng XU Gen-di LIU Wen-xiu GONG Chun-feng |
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| Institution: | College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004 |
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| Abstract: | Experiments were performed with two soybean cultivars (Zhuchun No.3 and Liaoxian No.3) under aeroponic culture. The objective was to investigate (1) The production and development of border cells in soybean; (2) The effect of Al3+ on the release and viability of border cells. Under aeroponic culture, border cells shape of both soybean cultivars at the initial stage was spherical and small. As cells developed, they became ellipsoidal or spectacular and long in shape. The number of border cells reached the maximum value (Zhuchun No.3 3000 and Lianxian No.3 3800 ) when soybean was 15-25 mm in length. The number and viability of border cells released from roots treated with 100, 200, 300, 400?mol·L-1 Al3+ were less than that from roots without Al3+ treatment, and Al3+ seriously inhibited the production and viability with high concentration Al3+ treatment. The PME activity increased with 200?mol·L-1 Al3+and 300?mol·L-1 Al3+ for 4h, whereas remarkably decreased with 400?mol·L-1 Al3+. These results suggested that high concentration Al3+ inhibited the production and release of soybean, decreased PME activity. Response of border cells to Al3+ in Liaoxian No.3 was more sensible than in Zhechun No.3. |
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| Keywords: | Soybean Root border cells Development Al~(3 ) toxicity |
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