| 浮萍中一个新rbcS基因启动子的克隆及分析 |
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| 引用本文： | 王友如.浮萍中一个新rbcS基因启动子的克隆及分析[J].分子植物育种,2010,8(1):41-44. |
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| 作者姓名： | 王友如 |
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| 作者单位： | 湖北师范学院生命科学院基因工程实验室,黄石,430052 |
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| 摘 要： | 为了分离组织特异性、诱导性的启动子,本研究根据新克隆的浮萍rbcS基因序列设计引物,采用改进的5'walking技术从浮萍基因组中克隆了一个新的rbcS基因启动子,命名为SSU5C基因启动子。序列分析表明:SSU5C基因启动子长度为1543bp,含有保守性元件TATA和CAATbox,并且含有水杨酸、脱落酸、赤霉素、茉莉酸甲酯等植物激素和葡萄糖等的保守顺式作用元件。Insilico分析初步推测SSU5C基因启动子受多种信号途径的协调调控。
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| 关 键 词： | 浮萍（Lemna gibba） rbcS基因 启动子 克隆 |
Analysis of a Novel rbcS Promoter Cloned in Duckweed |
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| Wang Youru.Analysis of a Novel rbcS Promoter Cloned in Duckweed[J].Molecular Plant Breeding,2010,8(1):41-44. |
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| Authors: | Wang Youru |
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| Institution: | Wang Youru Genetic Engineering Laboratory,College of Life Sciences,Hubei Normal University,Huangshi,430052 |
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| Abstract: | In order to isolate the tissue-specific and inducible promoter,we designed primers based on the DNA sequence of a newly cloned rbcS gene in duckweed,and cloned a novel rbcS promoter by using improved 5' walking technique,named as SSU5C promoter.Sequence analysis showed that the length of SSU5C promoter is 1 543 bp,it consisted of TATA and CAAT box,as well as cis-acting element of plant hormone,such as SA,ABA,GA,SA,and glucose etc.We preliminary predicted by in silico ananlysis that SSU5C promoter might be c... |
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| Keywords: | Duckweed (Lemna gibba) rbcS gene Promoter Cloning |
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