| 马铃薯腐烂茎线虫生防细菌的筛选与鉴定 |
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| 引用本文： | 张妞,王东,赵远征,项鹏,邱廷艳,赵鑫,高腾达,高志超,周洪友.马铃薯腐烂茎线虫生防细菌的筛选与鉴定[J].中国农学通报,2021,37(18):138-146. |
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| 作者姓名： | 张妞 王东 赵远征 项鹏 邱廷艳 赵鑫 高腾达 高志超 周洪友 |
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| 作者单位： | 1.内蒙古农业大学园艺与植物保护学院,呼和浩特 010018;2.内蒙古自治区农牧业科学院植物保护研究所,呼和浩特 010031;3.黑龙江省农业科学院黑河分院,黑龙江黑河 164312;4.内蒙古喀喇沁旗农牧局植保植检站,内蒙古喀喇沁旗 024409 |
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| 基金项目： | 内蒙古农业大学高层次人才科研启动项目“植物线虫病害微生态调控”(NDYB2018-53);内蒙古自治区自然科学基金“枯草芽胞杆菌ZWZ-19挥发性物质抑制腐烂茎线虫的分子机制研究”(2020BS03034);内蒙古自治区科技成果转化专项“微生态制剂的创制及其防控马铃薯土传病害的示范推广”(2019CG026) |
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| 摘 要： | 本研究旨在开发杀线虫活性高的生防资源以防控马铃薯腐烂茎线虫病。以34个细菌菌株发酵液为试材,通过直接触杀法测定其对马铃薯腐烂茎线虫的触杀活性;利用16S rDNA基因,并结合菌落形态和生理生化特征,确定生防菌株的分类地位。筛选出9个细菌菌株对马铃薯腐烂茎线虫的触杀活性较高,48 h线虫的校正死亡率达72.36%~82.59%;经鉴定,菌株Chi9-7、Nong3-5、Nong5-6和b1-3为枯草芽胞杆菌,菌株b5-4为贝莱斯芽胞杆菌,菌株Chi9-2、Xia4-11、Gu4-7和Z-1为解淀粉芽胞杆菌。本研究筛选并鉴定了杀线虫作用明显的9株芽胞杆菌,为微生物杀线剂的研发提供了新的生防资源,也为马铃薯腐烂茎线虫病的有效防控奠定了基础。
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| 关 键 词： | 马铃薯腐烂茎线虫 芽胞杆菌 触杀活性 生理生化特征 16S rDNA |
| 收稿时间： | 2020-10-30 |
Biocontrol Bacteria Against Ditylenchus destructor: Screening and Identification |
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| Zhang Niu,Wang Dong,Zhao Yuanzheng,Xiang Peng,Qiu Tingyan,Zhao Xin,Gao Tengda,Gao Zhichao,Zhou Hongyou.Biocontrol Bacteria Against Ditylenchus destructor: Screening and Identification[J].Chinese Agricultural Science Bulletin,2021,37(18):138-146. |
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| Authors: | Zhang Niu Wang Dong Zhao Yuanzheng Xiang Peng Qiu Tingyan Zhao Xin Gao Tengda Gao Zhichao Zhou Hongyou |
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| Institution: | 1.College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010018;2.Institute of Plant Protection, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031;3.Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe Heilongjiang 164312;4.Plant Protection and Plant Quarantine Station, Agricultural and Pastoral Bureau of Karakqin Banner, Karakqin Banner Inner Mongolia 024409 |
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| Abstract: | The objective is to develop bioagent resources with high nematicidal activity to control Ditylenchus destructor. Fermentation broths of 34 bacterial strains were used to test their contact activities against D. destructor by direct contact method. The classification status of biocontrol bacteria were identified based on colony phenotype, physiological and biochemical characteristics and 16S rDNA gene sequence. The results showed that 9 biocontrol bacteria with high contact activity were screened and could lead to D. destructor with 72.36%-82.59% corrected mortality after being treated with their fermentation broths for 48 h. Strains Chi9-7, Nong3-5, Nong5-6 and b1-3 were identified as Bacillus subtilis. b5-4 was B. velezensis, and Chi9-2, Xia4-11, Gu4-7 and Z-1 were B. amyloliquefaciens. In this study, 9 Bacillus strains with obvious nematicidal effect were screened and identified, which could provide novel biocontrol resources for the development of microbial nematicides and lay a foundation for the effective control of D. destructor. |
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| Keywords: | Ditylenchus destructor Bacillus spp contact activity physiological and biochemical characteristics 16S rDNA |
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