| 糖盐介质中γ-射线辐照对英诺克李斯特菌的杀菌效果研究 |
| |
| 引用本文： | 徐毓谦,李淑荣,李文慧,王丽,黄广学,汪慧华,马长路.糖盐介质中γ-射线辐照对英诺克李斯特菌的杀菌效果研究[J].核农学报,2021,35(5):1113-1120. |
| |
| 作者姓名： | 徐毓谦 李淑荣 李文慧 王丽 黄广学 汪慧华 马长路 |
| |
| 作者单位： | 1北京农业职业学院,北京 102442;2宁夏大学食品与葡萄酒学院,宁夏 银川 750021 |
| |
| 基金项目： | 国家科技支撑课题(2014BAA03B05),北京市职业院校教师素质提升计划食品营养与检测专业创新团队项目支持 |
| |
| 摘 要： | 为研究食品基质对γ-辐照杀菌效果的影响,本试验以英诺克李斯特菌(Listeria Innocua)为试材,采用lgN-D线性回归分析,建立活菌数-剂量的拟合方程,获得D10 值;利用D10值表征无菌水环境下γ-射线和电子束辐照的吸收剂量差异,以及不同γ-射线吸收剂量、糖浓度、盐浓度对英诺克李斯特菌杀菌效果的影响。结果表明,当辐照在无菌水液体环境下时,γ-射线的D10值均小于电子束,分别为0.788和0.872 kGy,表明γ-射线的杀菌效果更好;在γ-射线辐照下,杀灭生理盐水(0.85% NaCI)中英诺克李斯特菌的D10值与无菌水无明显差别,随着盐浓度的增加,D10值呈先增加后减少的趋势,其中在3%和5%盐浓度下的D10值分别为0.709和0.730 kGy,明显低于无菌水组;杀灭糖溶液中英诺克李斯特菌的D10值明显高于无菌水组,随着糖浓度的增加,D10值先升高后降低,其中5%糖溶液的D10值最大,为1.133 kGy。说明盐溶液对杀菌有协同作用,而糖溶液对杀菌有抵抗作用。本研究结果为进一步探索辐照杀菌机制,降低辐照剂量成本,提升产品品质提供了一定的参考。
|
| 关 键 词： | 英诺克李斯特菌 辐照杀菌 电子束 辐照环境 D10值 |
| 收稿时间： | 2020-11-03 |
The γ-Irradiation Sterilization Effect on Listeria Innocua in Sugar and Salt Medium |
| |
| XU Yuqian,LI Shurong,LI Wenhui,WANG Li,HUANG Guangxue,WANG Huihua,MA Changlu.The γ-Irradiation Sterilization Effect on Listeria Innocua in Sugar and Salt Medium[J].Acta Agriculturae Nucleatae Sinica,2021,35(5):1113-1120. |
| |
| Authors: | XU Yuqian LI Shurong LI Wenhui WANG Li HUANG Guangxue WANG Huihua MA Changlu |
| |
| Institution: | 1Beijing Vocational College of Agriculture, Beijing 102442;2School of Food and Wine, Ningxia University, Yinchuan, Ningxia 750021 |
| |
| Abstract: | In order to know the effect of food matrix on the sterilization efficacy of γ-irradiation. By lgN-D linear regression analysis, the formula of the number of viable bacteria was obtained. The D10 value was used to characterize the difference of absorbed dose of gamma ray and electron beam irradiation in sterile water environment, and the influence of different gamma ray absorption dose, sugar concentration and salt solution concentration on the germicidal efficacy of Listeria Innocua was analyzed. The results shows that when the irradiation environment is the sterile water, the gamma ray D10 value is less than the electron beam, respectively 0.788 and 0.872 kGy, which shows that the gamma ray sterilization effect is better. Under the irradiation of gamma ray, The D10 value of Listeria Innocua in sterilized saline (0.85% NaCI) was not significantly different from that of sterile water. With the increase of salt concentration, the D10 value of innocua first increased and then decreased, and the D10 value at 3% and 5% salt concentration was 0.709 kGy and 0.730 kGy, respectively, lower than that of sterile water. The D10 value of Listeria Innocua in sugar solution was significantly higher than that of sterile water. With the increase of the concentration of sugar solution, D10 first increased and then decreased. The D10 value of 5% sugar solution was the largest, which was 1.13 kGy. It could be seen that salt solution has a synergistic effect on sterilization, while sugar solution has a resistance effect on sterilization. The results provide a reference for further exploring the mechanism of irradiation sterilization, reducing radiation dose cost and improving product quality. |
| |
| Keywords: | Listeria Innocua irradiation microorganism inactivation electron beam irradiation environment D10 value |
|
| 点击此处可从《核农学报》浏览原始摘要信息 |
| 点击此处可从《核农学报》下载免费的PDF全文 |
|
