| 3种植物提取物对稻瘟病菌的抑菌活性及其抑菌机理研究 |
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| 引用本文： | 梁琪,付歆崴,李云鹏,杨胜甫.3种植物提取物对稻瘟病菌的抑菌活性及其抑菌机理研究[J].中国农学通报,2021,37(15):120-127. |
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| 作者姓名： | 梁琪 付歆崴 李云鹏 杨胜甫 |
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| 作者单位： | 黑龙江大学现代农业与生态环境学院,哈尔滨 150080 |
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| 基金项目： | 国家自然科学基金“大兴安岭林下调落物慢速生长产孢真菌的隐秘代谢物及其抗菌活性”(31870528);黑龙江省科学基金资助项目“野生茄子托鲁巴姆叶片抑菌活性成分及控病机制研究”(QC2014C024) |
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| 摘 要： | 通过探讨黄连、甘草和肉桂提取物对稻瘟病菌的抑制效果及作用机理,为开发新型稻瘟病菌抑制剂奠定基础。以菌丝生长速率法测定抑菌活性,室内常规分析方法观测菌丝细胞膜通透性、物质合成与代谢和超显微形态。结果表明,3种提取物对稻瘟病菌菌丝生长均具有较强的抑制活性,黄连、甘草、肉桂的半抑菌浓度依次为4.17 mg/mL、4.48 mg/mL和5.22 mg/mL;经3种提取物作用后,稻瘟病菌菌丝的丙二醛含量和外渗电导率升高、丙酮酸和可溶性蛋白含量降低,菌丝出现畸形等生长异常现象。由此可见,3种提取物抑制稻瘟病菌效果突出,可破坏菌丝细胞膜和超显微形态,干扰物质合成与代谢,有望开发为稻瘟病菌抑制剂。
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| 关 键 词： | 水稻病害 稻瘟病菌 抑菌活性 抑菌机理 植物提取物 黄连 甘草 肉桂 |
| 收稿时间： | 2020-09-04 |
Three Kinds of Plant Extracts Against Pyricularia grisea: Antifungal Activity and Antifungal Mechanism |
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| Liang Qi,Fu Xinwei,Li Yunpeng,Yang Shengfu.Three Kinds of Plant Extracts Against Pyricularia grisea: Antifungal Activity and Antifungal Mechanism[J].Chinese Agricultural Science Bulletin,2021,37(15):120-127. |
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| Authors: | Liang Qi Fu Xinwei Li Yunpeng Yang Shengfu |
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| Institution: | College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080 |
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| Abstract: | The aims are to explore the inhibitory effect and mechanism of Coptis chinensis, Glycyrrhiza uralensis and Cinnamomum cassia extracts on Pyricularia grisea, and lay a theoretical foundation for developing new inhibitors of Pyricularia grisea. The antifungal activity was determined by the hypha growth rate method. The cell membrane permeability of hyphae, material synthesis and metabolism, and ultra microstructure of hyphal cells were observed by the conventional indoor analysis method. The results showed that the three extracts had strong inhibitory activity on the mycelial growth of Pyricularia grisea. The half fungal inhibitor concentration of Coptis chinensis, Glycyrrhiza uralensis and Cinnamomum cassia were 4.17 mg/mL, 4.48 mg/mL and 5.22 mg/mL, respectively. After treated with the three extracts, malondialdehyde content and conductivity leakage of Pyricularia grisea hypha increased, and the contents of pyruvate and soluble protein decreased, and the hyphae showed morphological changes and other abnormal growth. It can be seen that the three extracts have outstanding inhibitory effect on Pyricularia grisea, and they can destroy the cell membrane and ultramicroscopic morphology and interfere with the synthesis and metabolism of substances, which are expected to be developed as inhibitors of Pyricularia grisea. |
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| Keywords: | rice disease: Pyricularia grisea antifungal activity antifungal mechanism plant extract Coptis chinensis Glycyrrhiza uralensis Cinnamomum cassia |
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