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| 葡萄内生细菌的激活及其对霜霉病菌的抑制作用 | |
| 引用本文： | 史晓梦,张书蔚,王亚聪,申红妙,冉隆贤.葡萄内生细菌的激活及其对霜霉病菌的抑制作用[J].植物病理学报,2021,51(6):951-961. |
| 作者姓名： | 史晓梦 张书蔚 王亚聪 申红妙 冉隆贤 |
| 作者单位： | 河北农业大学林学院,保定 071000; 河北经贸大学,石家庄 050061; 河北省林木种质资源与森林保护重点实验室,保定 071000 |
| 基金项目： | 国家重点研发计划(2018YFD0201300);国家公益性行业(农业)科研专项(201203035) |
| 摘 要： | 为了探究化学诱导剂诱导葡萄抗霜霉病的特性与葡萄内生菌激活的关系,筛选出对葡萄霜霉病有较高生防效果的菌株,本研究通过光学显微镜、扫描电子显微镜观察和传统培养法,检测了葡萄茎段的内生菌,并应用4种化学诱导剂对葡萄叶片和茎段进行处理,对被激活的内生菌进行分离和生防效果的测定。结果显示,在光学显微镜和扫描电子显微镜下均可观察到葡萄茎段内存在多种不同形态的内生细菌,但均处于不可培养状态;经不同浓度的诱导剂处理后叶片和茎段内生菌均增多,其中均以β-氨基丁酸(β-Aminobutyric acid, BABA)激活菌株数最多,诱导叶片和带叶茎段抗霜霉病效果最好,分别为80.4%和86.8%;被激活的叶片和茎段内生菌均为细菌,且多数为革兰氏阳性细菌,只有2株为革兰氏阴性细菌,其中,由BABA激活的革兰氏阴性细菌L-B-4生防效果高达97.2%,显著高于其他菌株,结合形态特征与16S rDNA序列分析,菌株L-B-4被鉴定为产氮假单胞杆菌(Pseudomonas azotoformans)。本文首次发现化学诱导剂在诱导葡萄抗霜霉病的过程中对葡萄内生细菌有激活作用,且被激活菌株对葡萄霜霉病有较高的生防效果。 |
| 关 键 词： | 生物防治 内生细菌 葡萄霜霉病 诱导抗病性 激活 |
| 收稿时间： | 2020-11-03 |
Resuscitation of grape endophytic bacteria and their inhibitive effects against Plasmopara viticola |
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| SHI Xiao-meng,ZHANG Shu-yu,WANG Ya-cong,SHEN Hong-miao,RAN Long-xian.Resuscitation of grape endophytic bacteria and their inhibitive effects against Plasmopara viticola[J].Acta Phytopathologica Sinica,2021,51(6):951-961. | |
| Authors: | SHI Xiao-meng ZHANG Shu-yu WANG Ya-cong SHEN Hong-miao RAN Long-xian |
| Institution: | College of Forestry, Hebei Agricultural University, Baoding 071000, China; Hebei University of Economics and Business, Shijiazhuang 050061, China; Key Laboratory of Forest Germplasm Resources and Forest Protection in Hebei Province, Baoding 071000, China |
| Abstract: | This research focused on the relationship between induced resistance of grape by different chemical elicitors to grape downy mildew and resuscitated endophytes, and screening potential strains with higher biocontrol efficacy against grape downy mildew. Endophytes in grape leaves and stems were detected using traditional culture, optical microscope and scanning electron microscope (SEM). The leaves and stems of grape were treated with 4 chemical elicitors at different concentrations, including β-aminobutyric acid (BABA), 2,1,3-benzothiadiazole (BTH), salicylic acid (SA) and gibberellic acid (GA3). The resuscitated endophytes were isolated and their biocontrol efficacy were performed on detached grape leaves. The results showed that there were many endophytic bacteria with different morphology by observation under optical microscope and SEM, but none of them were culturable. After the treatments of different concentration of elicitors, the number of resuscitated bacterial isolates in grape leaves and stems increased, in which BABA performed the best resuscitating effect on endophytic bacteria and exhibited the highest induced efficacy against grape downy mildew, reaching 80.4% and 86.8% in leaves and stems with leaves, respectively. The resuscitated endophytes were all bacteria, in which most of them were Gram-positive bacteria, and only two strains belong to Gram-negative bacteria. The biocontrol efficacy of Gram-negative bacterium L-B-4 resuscitated by BABA was as high as 97.2%, which was significantly higher than other strains. Based on the characteristics in morphology and sequence analysis of 16S rDNA, the strain L-B-4 was identified as Pseudomonas azotoformans. To our knowledge, this is the first report that grape endophytic bacteria could be resuscitated by chemical elicitors during the inducing process of grape against downy mildew, and the biocontrol efficacy of resuscitated strains against grape downy mildew was significantly increased. |
| Keywords: | biocontrol endophytic bacteria grape downy mildew induced disease resistance resuscitation |
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