| 农大4号与DS-1欧李正、反交F1代果实品质的遗传变异分析 |
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| 引用本文： | 付鸿博,王鹏飞,徐豆,穆霄鹏,张建成,付宝春,杜俊杰.农大4号与DS-1欧李正、反交F1代果实品质的遗传变异分析[J].核农学报,2021,35(10):2223-2233. |
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| 作者姓名： | 付鸿博 王鹏飞 徐豆 穆霄鹏 张建成 付宝春 杜俊杰 |
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| 作者单位： | 1山西农业大学园艺学院,山西 太谷 0308012山西农业大学果树研究所,山西 太谷 030801 |
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| 基金项目： | 山西农业大学生物育种工程项目(YZGC108);山西省“1331工程”标志性成果奖补项目晋欧1号(J201911313);山西省重点研发计划重点项目(201703D211001-04-04);山西省应用基础研究项目(201801D121251) |
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| 摘 要： | 为探究欧李果实品质的遗传规律,本研究选用欧李农大4号与DS-1及其正、反交后代群体为试验材料,测定双亲及后代群体果实的平均单果重、核重、纵横径、果形指数、可溶性固形物含量、类黄酮含量、总酚含量及抗氧化能力(DPPH、FRAP、ABTS),对其后代遗传规律进行分析。结果表明,所测定的指标均呈现正态或偏正态分布,呈连续变异,为多基因控制的数量遗传性状。纵径、横径、果形指数和可溶性固形物含量的变异系数均小于20%,后代未出现广泛分离,选择潜力较小,其中果形指数的变异系数最小,选择潜力最小,这与大多数欧李果实呈现扁圆形的情况一致。平均单果重、核重、类黄酮含量、总酚含量和抗氧化能力的变异系数均超过20%,后代广泛分离,选择潜力较大,尤其以平均单果重的选择潜力最大。平均单果重、纵横径受父本影响较大,以大果为父本时更易选出大果后代。果形指数不受父母本影响。可溶性固形物含量和核重表现明显的衰退变异,为趋小性遗传。以高类黄酮、总酚含量和抗氧化能力的植株为母本时,后代更易选出高类黄酮、总酚含量和抗氧化能力的优株。相关性分析将表型特征与内在物质含量相联系,为初步判断物质含量奠定重要基础。主成分分析提取了包括清除ABTS自由基和类黄酮含量的第一主成分和包括纵横径的第二主成分。聚类分析筛选出了具有较高生物活性、较强抗氧化能力和果实较大等不同特征的优株,可根据育种目标加以重点利用。本研究为欧李育种提供了重要参考的意义。
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| 关 键 词： | 欧李 类黄酮 抗氧化能力 杂交育种 遗传分析 |
| 收稿时间： | 2020-06-23 |
Heredity and Variation Analysis of Fruit Quality in the F1 Generation From Reciprocal Crosses Between Nongda 4 and DS-1 Chinese Dwarf Cherry (Cerasus humilis) |
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| FU Hongbo,WANG Pengfei,XU Dou,MU Xiaopeng,ZHANG Jiancheng,FU Baochun,DU Junjie.Heredity and Variation Analysis of Fruit Quality in the F1 Generation From Reciprocal Crosses Between Nongda 4 and DS-1 Chinese Dwarf Cherry (Cerasus humilis)[J].Acta Agriculturae Nucleatae Sinica,2021,35(10):2223-2233. |
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| Authors: | FU Hongbo WANG Pengfei XU Dou MU Xiaopeng ZHANG Jiancheng FU Baochun DU Junjie |
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| Institution: | 1College of Horticulture, Shanxi Agriculture University, Taigu, Shanxi 0308012Research Institute of Pomology, Shanxi Agriculture University, Taigu, Shanxi 030801 |
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| Abstract: | In order to explore the genetic of fruit quality and provide a reference for breeding. In this study, Chinese dwarf cherry Nongda 4, DS-1 and their reciprocal crosses population were selected as the experimental materials to test the mean single fruit weight, stone weight, diameter and length, index of fruit shape, contents of soluble solids, total flavonoid and total phenol, and the antioxidant capacity(DDPH、FRAP、ABTS). The results showed that all the indexes showed normal or partial normal distribution with continuous variation, and were quantitative genetic characters controlled by multiple genes. The coefficient variations of vertical diameter, horizontal diameter, fruit shape index and soluble solid content were all less than 20%, indicating narrow separation and low selection potential, among which the coefficient variation of fruit shape index was the smallest, and the selection potential was the lowest, shown consistent with the oblate shape of most fruits. The coefficient variation of single fruit weight, stone weight, total flavonoid content, total phenol content and antioxidant capacity were all larger than 20%, with wide separation and great selection potential, especially single fruit weight. The single fruit weight, vertical diameter and horizontal diameter were greatly affected by the male parent, and it was easier to select offspring with big fruit when the male parent had big fruits. The fruit shape index was not affected by parents. The soluble solid content and stone weight showed obvious decaying variation, had an inheritance of decreasing tendency. When the female parent had high total flavonoid content, total phenol content and antioxidant capacity, it was more likely to select the superior offspring with high total flavonoid content, total phenol content and antioxidant capacity. Correlation between the phenotypic characteristics and the intrinsic substance content gives an important reference for the preliminary determination of substance content. Principal component analysis extracted the first principal component including ABTS free radical scavenging and total flavonoid content, and the second principal component including the vertical and horizontal diameter. The superior plants with high biological activity, strong antioxidant capacity and large fruit were selected by cluster analysis, which can be used for different breeding objectives and provide a reference for breeding. |
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| Keywords: | Chinese dwarf cherry flavonoid antioxidant capacity hybridization breeding genetic analysis |
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