| 建佛手总黄酮的超声波提取工艺优化及抗氧化分析 |
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| 引用本文： | 黄靖,陈婵,黄晓梅.建佛手总黄酮的超声波提取工艺优化及抗氧化分析[J].中国农学通报,2021,37(7):126-131. |
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| 作者姓名： | 黄靖 陈婵 黄晓梅 |
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| 作者单位： | 福建农业职业技术学院,福州 350007 |
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| 基金项目： | 福建农业职业技术学院科研课题“建佛手的综合开发利用研究”(2018JS014) |
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| 摘 要： | 为研究福建佛手总黄酮的超声波提取技术及其抗氧化性,通过正交实验法确定超声波提取建佛手总黄酮的最优提取工艺,即在超声频率为45 kHz与80 kHz双频模式下,在料液比1:35、乙醇浓度60%、超声功率50 W、提取温度50℃下提取80 min,建佛手总黄酮的提取率最高,可达3.33%。体外抗氧化实验表明,建佛手总黄酮对·OH及O2-·均有明显的清除作用,但对·OH及对O2-·的清除作用均不如同浓度的Vc溶液。当建佛手总黄酮质量浓度为1.4 mg/mL时,佛手总黄酮对·OH和O2-·的清除率分别为54.4%和39.2%。
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| 关 键 词： | 佛手 总黄酮 超声波 提取 抗氧化 |
| 收稿时间： | 2020-05-13 |
Flavonoids from Fujian Bergamot: Ultrasonicly Extracting Technology and Its Antioxidation Properties |
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| Huang Jing,Chen Chan,Huang Xiaomei.Flavonoids from Fujian Bergamot: Ultrasonicly Extracting Technology and Its Antioxidation Properties[J].Chinese Agricultural Science Bulletin,2021,37(7):126-131. |
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| Authors: | Huang Jing Chen Chan Huang Xiaomei |
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| Institution: | Fujian Vocational College of Agriculture, Fuzhou 350007 |
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| Abstract: | The paper aims to optimize the extraction process of flavonoids from Fujian bergamot by ultrasonicly method and study its antioxidation activity. The extraction process condition optimized by orthogonal experiments is: under the double frequency of ultrasonic frequency of 45 kHz and 80 kHz, the ratio of material to liquid of 1:35 (g:mL), the ethanol concentration of 60%, the ultrasonic power and ultrasonic temperature of 50 W and 50℃ respectively, and the extraction time of 80 min, the extraction yield of flavonoids could reach the highest of 3.33%. The scavenging effect of Fujian bergamot flavonoids on ·OH and O2-·are obvious and effective, but the antioxidant activity is weaker than that of Vc. When the solubility of Fujian bergamot flavonoids is 1.4 mg/mL, the scavenging rate is 54.4% and 39.2%, respectively. |
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| Keywords: | bergamot flavonoids ultrasound extraction antioxidation activity |
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