| 番茄产后灰霉病的病原鉴定及生物防治 |
| |
| 引用本文： | 沈艳,何鹏搏,何鹏飞,吴毅歆,孔宝华,李兴玉,Shahzad Munir,何月秋.番茄产后灰霉病的病原鉴定及生物防治[J].中国农学通报,2021,37(13):102-107. |
| |
| 作者姓名： | 沈艳 何鹏搏 何鹏飞 吴毅歆 孔宝华 李兴玉 Shahzad Munir 何月秋 |
| |
| 作者单位： | 云南农业大学省部共建云南生物资源保护与利用国家重点实验室,昆明 650201 |
| |
| 基金项目： | 科技部国际合作计划项目“防治植物病害和促进植物生长的微生物开发应用及机制研究”(2009DFA32360) |
| |
| 摘 要： | 为探索生物防治番茄产后灰霉病,采用柯赫氏法则从罹病的番茄果实上分离得到菌株FQ-2,基于形态学和rDNA-ITS序列,对该菌株进行病原鉴定,采用小麦内生菌解淀粉芽孢杆菌 B9601-Y2(Y2)和球花石斛内生菌贝莱斯芽孢杆菌DJB5(DJB5)对番茄灰霉病进行生物防治。FQ-2被鉴定为灰葡萄孢菌(Botrytis cinerea),且Y2和DJB5对番茄产后果实灰霉病均有较好的预防和治疗效果,Y2 1×107 cfu/mL和DJB5 1×108 cfu/mL悬浮剂在处理当天接种FQ-2,10天后的防效分别达88.10%和73.47%。在接种灰霉病菌当天再接种1×108cfu/mL的Y2和1×107 cfu/mL的DJB5,10天后灰霉病的防治效果分别达68.56%和78.74%。研究结果对番茄产后灰霉病的生物防治具有参考作用。
|
| 关 键 词： | 番茄 生物防治 灰霉病 灰葡萄孢菌 解淀粉芽孢杆菌 贝莱斯芽孢杆菌 病原鉴定 |
| 收稿时间： | 2020-12-16 |
Pathogen Identification and Biological Control of Gray Mold on Postharvest Tomato |
| |
| Shen Yan,He Pengbo,He Pengfei,Wu Yixin,Kong Baohua,Li Xingyu,Shahzad Munir,He Yueqiu.Pathogen Identification and Biological Control of Gray Mold on Postharvest Tomato[J].Chinese Agricultural Science Bulletin,2021,37(13):102-107. |
| |
| Authors: | Shen Yan He Pengbo He Pengfei Wu Yixin Kong Baohua Li Xingyu Shahzad Munir He Yueqiu |
| |
| Institution: | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Yunnan Agricultural University, Kunming 650201 |
| |
| Abstract: | In order to explore the biological control of gray mold caused by Botrytis cinerea on postharvest tomato, strain FQ-2 was isolated from tomato fruit by the Koch’s postulate, and the pathogen was identified based on its morphology and rDNA-ITS sequence. Bacillus amyloliquefaciens B9601-Y2 (Y2), an endophyte of wheat and B. velezensis DJB5 (DJB5), an endophyte of Dendrobium thyrsiflorum were used for biological control of the disease. By analyses, FQ-2 was identified as Botrytis cinerea, Y2 and DJB5 had good preventive and therapeutic effects on gray mold of tomato. Y2 with 1×107cfu/mL and DJB5 with 1×108cfu/mL had 88.10% and 73.47% of prevention effects, respectively, after 10 days when they were inoculated following FQ-2 in the same day. In the curing experiment, on the day of inoculation of B. cinerea, Y2 with 1×108cfu/mL and DJB5 with 1×107cfu/mL had 68.56% and 78.74% of curing effect, respectively, after 10 days when they were inoculated following FQ-2 in the same day. These results are of great significance to the postharvest biological control of tomato gray mold. |
| |
| Keywords: | tomato biological control gray mold Botrytis cinerea Bacillus amyloliquefaciens Bacillus velezensis pathogen identification |
|
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
