| 石蒜鳞茎膨大过程中碳水化合物代谢相关基因的差异表达研究 |
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| 引用本文： | 许俊旭,李青竹,李叶,杨柳燕,张永春,蔡友铭.石蒜鳞茎膨大过程中碳水化合物代谢相关基因的差异表达研究[J].核农学报,2021,35(10):2250-2257. |
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| 作者姓名： | 许俊旭 李青竹 李叶 杨柳燕 张永春 蔡友铭 |
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| 作者单位： | 1上海市农业科学院林木果树研究所,上海 2014032上海市金山区廊下镇农业技术推广服务站,上海 201516 |
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| 基金项目： | 上海市科技兴农重点攻关项目(沪农科攻字(2016)第6-1-6号);上海市科学技术委员会研发平台建设项目(18DZ2291400) |
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| 摘 要： | 为探讨石蒜Lycoris radiata (L’Her.) Herb.]鳞茎膨大过程中碳水化合物积累的变化规律,本研究依据前期已构建的材料,分析了石蒜鳞茎膨大过程中碳水化合物含量、相关酶活性以及碳水化合物代谢相关基因的表达变化。结果表明,随着石蒜鳞茎的膨大,蔗糖含量不断升高,蔗糖代谢酶SUS以及淀粉合成酶AGPase、SSS和GBSS的活性不断增强,从而加快淀粉的合成,这一过程可能受到编码蔗糖代谢及淀粉合成相关酶基因的正向调控,如SUS1、SUS2、SUS4、UGPA、AGPS2、AGPL2、SS2、SS3、GBSS1、SBE1、SBE2、SBE3等。另外,本研究还发现分别在AMY3和BAMY7、BAMY8、BAMY9基因的调控作用下,石蒜鳞茎膨大过程中α-淀粉酶和β-淀粉酶的活性也增强,从而加速淀粉的分解,提高鳞茎中可溶性糖含量,并可能在糖转运相关基因的作用下加快向鳞茎发育部位的转运,为鳞茎的后续膨大过程提供能量。本研究初步揭示了石蒜鳞茎膨大过程中碳水化合物代谢活动的变化规律,并挖掘到一些可能在此过程中发挥重要作用的调控基因,为后续利用分子生物学等手段加速石蒜鳞茎的膨大提供了理论依据。
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| 关 键 词： | 石蒜 鳞茎膨大 碳水化合物代谢 基因表达 |
| 收稿时间： | 2020-07-13 |
Differential Expression of Genes Related to Carbohydrate Metabolism During Bulb Development in Lycoris radiata |
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| XU Junxu,LI Qingzhu,LI Ye,YANG Liuyan,ZHANG Yongchun,CAI Youming.Differential Expression of Genes Related to Carbohydrate Metabolism During Bulb Development in Lycoris radiata[J].Acta Agriculturae Nucleatae Sinica,2021,35(10):2250-2257. |
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| Authors: | XU Junxu LI Qingzhu LI Ye YANG Liuyan ZHANG Yongchun CAI Youming |
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| Institution: | 1Forestry and Pomology Research Institute, Shanghai Academy of Agriculture Sciences, Shanghai 2014032Agricultural Technology Extension Service Station, Langxia Town, Jinshan District, Shanghai 201516 |
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| Abstract: | In order to study the changes in carbohydrate accumulation during bulb development process in Lycoris radiata, we investigated the changes in carbohydrate content, starch synthesis and metabolic enzyme activity and the expression patterns of genes involved in carbohydrate metabolism in developing bulbs. The results showed that sucrose content, the activities of sucrose metabolism enzyme SUS and starch synthesis enzymes AGPase, SSS and GBSS were increased, resulting in the acceleration of starch synthesis, which may be positively regulated by several genes such as SUS1, 2, 4, UGPA, AGPS2, AGPL2, SS2, 3, GBSS1 and SBE1, 2, 3. In addition, the activities of starch metabolism enzymes were also enhanced under the regulation of AMY3 and BAMY7, 8, 9 genes, which resulting in the acceleration of starch degradation and the increasement of soluble sugar content in the bulbs. Sugars may be transferred to the developing bulb to provide energy for subsequent development under the control of several sugar transport-related genes. This study revealed the changes of carbohydrate metabolism during bulb development of Lycoris radiata, and identified several genes that may play important roles in this process, which could provide a theoretical basis for accelerating bulb development by molecular biology approaches. |
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| Keywords: | Lycoris radiata bulb development carbohydrate metabolism gene expression |
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